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Gene Expression Data
Assay Details
Assay
Reference: J:146829 Yamada W, et al., Craniofacial malformation in R-spondin2 knockout mice. Biochem Biophys Res Commun. 2009 Apr 10;381(3):453-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7485762
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Fixative was periodate-lysine-paraformaldehyde.
Results
Specimen S1A: embryonic day 16.5 (more )
Note: Triple labeled: green - Acta2; red - Pecam1 (assay MGI:7485759); blue - Fn1 (assay MGI:7485771).
Structure Level Pattern Image Note
TS24: bronchiole Present S1A

Specimen S1B: embryonic day 16.5; Rspo2tm1Suda/Rspo2tm1Suda (more )
Note: Triple labeled: green - Acta2; red - Pecam1 (assay MGI:7485759); blue - Fn1 (assay MGI:7485771).
Structure Level Pattern Image Note
TS24: bronchiole Present S1B Expression in this mutant is the same as in wild-type.

Specimen S1C: embryonic day 18.5 (more )
Note: Triple labeled: green - Acta2; red - Pecam1 (assay MGI:7485759); blue - Fn1 (assay MGI:7485771).
Structure Level Pattern Image Note
TS26: bronchiole Present S1C

Specimen S1D: embryonic day 18.5; Rspo2tm1Suda/Rspo2tm1Suda (more )
Note: Triple labeled: green - Acta2; red - Pecam1 (assay MGI:7485759); blue - Fn1 (assay MGI:7485771).
Structure Level Pattern Image Note
TS26: bronchiole Present S1D Expression in this mutant is the same as in wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory