Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:51966 Sekine K, et al., Fgf10 is essential for limb and lung formation. Nat Genet. 1999 Jan;21(1):138-41
Assay type:	RNA in situ
MGI Accession ID:	MGI:7484048
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen 3c: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	3c	Expression is in the posterior limb bud mesoderm.

Specimen 3d: embryonic day 10.5; Fgf10^tm1Ska/Fgf10^tm1Ska (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Absent		3d

Specimen 3k: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	3k	Expression is in the posterior limb bud mesoderm.
TS17: hindbrain floor plate	Present		3k
TS17: notochord	Present		3k
TS17: forebrain	Present	Regionally restricted	3k	Expression is ventral.

Specimen 3l: embryonic day 10.5; Fgf10^tm1Ska/Fgf10^tm1Ska (more

)

Structure	Level	Image	Note
TS17: limb mesenchyme	Absent	3l
TS17: forebrain	Present	3l	Expression is this tissue is the same as in wild-type.
TS17: hindbrain floor plate	Present	3l	Expression is this tissue is the same as in wild-type.
TS17: notochord	Present	3l	Expression is this tissue is the same as in wild-type.

Specimen 5a: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: bronchus	Present	Regionally restricted	5a	Expression is in the endoderm.
TS19: trachea	Present	Regionally restricted	5a	Expression is in the endoderm.

Specimen 5b: embryonic day 11.5; Fgf10^tm1Ska/Fgf10^tm1Ska (more

)

Structure	Level	Pattern	Image	Note
TS19: trachea	Present		5b	Expression ends where the bronchi would have started.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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