Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104312
Li HP, et al., Aberrant trajectory of thalamocortical axons associated with abnormal localization of neurocan immunoreactivity in the cerebral neocortex of reeler mutant mice. Eur J Neurosci. 2005 Dec;22(11):2689-96
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7484009
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Gene symbol:
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Ncan
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Gene name:
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neurocan
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Antibody:
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anti-Neurocan
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Fixative was acid alcohol fixative (95% ethanol/5% acetic acid).
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Results
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Specimen
2E:
embryonic day 16.5; Relnrl/Reln +
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
2F:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3B:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neocortex |
Present |
Regionally restricted |
3B
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Expression is in horizontal axon bundles.
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Specimen
3C:
embryonic day 16.5; Relnrl/Reln +
(more )
Note: Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
Specimen
3C:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cortical subplate |
Present |
Regionally restricted |
3C
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Expression is in fibers.
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Specimen
3D:
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
Specimen
3D:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cortical subplate |
Present |
Regionally restricted |
3D
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Expression is in fibers.
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Specimen
3E:
embryonic day 16.5; Relnrl/Reln +
(more )
Note: Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
Specimen
3E:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
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Specimen
3F:
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
Specimen
3F:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Ncan; brown - L1cam (assay MGI:7482649).
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Specimen
4A:
embryonic day 15.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical subplate |
Present |
Regionally restricted |
4A
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Expression is in fibers.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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L1camtm1Mtei/Y
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical subplate |
Present |
Regionally restricted |
4B
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Expression is in fibers that were less fasciculated and more diffusely distributed compared to wild-type.
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Analysis Tools