Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104312
Li HP, et al., Aberrant trajectory of thalamocortical axons associated with abnormal localization of neurocan immunoreactivity in the cerebral neocortex of reeler mutant mice. Eur J Neurosci. 2005 Dec;22(11):2689-96
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7482649
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Gene symbol:
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L1cam
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Gene name:
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L1 cell adhesion molecule
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Antibody:
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anti-L1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Fixative was acid alcohol fixative (95% ethanol/5% acetic acid).
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Results
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Specimen
1C:
embryonic day 16.5; Relnrl/Reln +
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
1D:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3A:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neocortex |
Present |
Regionally restricted |
3A
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Expression is in axonal bundles obliquely transversing the neocortex.
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Specimen
3C:
embryonic day 16.5; Relnrl/Reln +
(more )
Note: Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
Specimen
3C:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
3C
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Expression is in fibers running through the internal capsule.
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| TS24: cerebral cortex |
Present |
Regionally restricted |
3C
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Expression is in fibers innervating the cortical anlage.
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| TS24: cortical subplate |
Present |
Regionally restricted |
3C
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Expression is in fibers.
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Specimen
3D:
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
Specimen
3D:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
3D
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Expression is in fibers running through the internal capsule.
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| TS24: cerebral cortex |
Present |
Regionally restricted |
3D
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Expression is in fibers innervating the cortical anlage.
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| TS24: cortical subplate |
Present |
Regionally restricted |
3D
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Expression is in fibers.
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| TS24: cortical plate |
Present |
Regionally restricted |
3D
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Expression is in fibers towards the superplate.
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Specimen
3E:
embryonic day 16.5; Relnrl/Reln +
(more )
Note: Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
Specimen
3E:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cortical subplate |
Present |
Regionally restricted |
3E
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Expression is in fibers.
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Specimen
3F:
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
Specimen
3F:
(close )
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Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - L1cam; purple - Ncan (assay MGI:7484009).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cortical subplate |
Present |
Regionally restricted |
3F
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Expression is in fibers.
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| TS24: cortical plate |
Present |
Regionally restricted |
3F
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Expression is in fibers towards the superplate.
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