Gfap Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:335425 Derbyshire ML, et al., Loss of Tbx3 in Mouse Eye Causes Retinal Angiogenesis Defects Reminiscent of Human Disease. Invest Ophthalmol Vis Sci. 2023 May 1;64(5):1
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7482276
Gene symbol:	Gfap
Gene name:	glial fibrillary acidic protein

Antibody:	anti-GFAP (clone G-A-5)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Results

S4A ©

Specimen S4A: postnatal day 26; Tbx3^tm3.1Moon/Tbx3⁺ (more

)
Note: Double labeled: red - Gfap; green - IB4 lectin.

Structure	Level	Pattern	Image	Note
TS28: retina internal limiting lamina	Present	Regionally restricted	S4A	Expression in dorsal inner limiting membrane.

S4B ©

Specimen S4B: postnatal day 26; Tbx3^tm3.1Moon/Tbx3^tm3.1Moon, Tg(Dkk3-cre)D9Tfur/0 (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - IB4 lectin.

Structure	Level	Pattern	Image	Note
TS28: retina internal limiting lamina	Present	Regionally restricted	S4B	Expression in fewer cells in dorsal inner limiting in membrane than in wild type.

3L ©

Specimen 3L: postnatal day 26; Tbx3^tm3.1Moon/Tbx3⁺ (more

)
Note: Double labeled: red - Gfap; green - IB4 lectin.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Mueller cell	Present		3L
TS28: retina ganglion cell layer	retinal astrocyte	Present		3L

3M ©

Specimen 3M: postnatal day 26; Tbx3^tm3.1Moon/Tbx3^tm3.1Moon, Tg(Dkk3-cre)D9Tfur/0 (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - IB4 lectin.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Mueller cell	Present		3M
TS28: retina ganglion cell layer	retinal astrocyte	Present		3M

5A ©

Specimen 5A: postnatal day 9; Tbx3^tm3.1Moon/Tbx3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	5A	Astrocytes reach edge of retina.

5B ©

Specimen 5B: postnatal day 9; Tbx3^tm3.1Moon/Tbx3⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retinal astrocyte	Present	Regionally restricted	5B	Astrocytes reach edge of retina.

5C ©

Specimen 5C: postnatal day 30; Tbx3^tm3.1Moon/Tbx3⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retinal astrocyte	Present	Regionally restricted	5C	Astrocytes reach edge of retina.

5D ©

5D' ©

5D'' ©

Specimen 5D: postnatal day 30; Tbx3^tm3.1Moon/Tbx3^tm3.1Moon, Tg(Dkk3-cre)D9Tfur/0 (more

)
Note: Conditional mutant. Double labeled: orange - Gfap; green - Pecam1 (assay MGI:7482270).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retinal astrocyte	Present	Regionally restricted	5D , 5D'' , 5D'	Astrocytes lack stereotypical lattice which fails to reach retinal edge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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