Lef1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:207609 Hilliard SA, et al., Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol. 2014 Mar 1;387(1):1-14
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7481928
Gene symbol:	Lef1
Gene name:	lymphoid enhancer binding factor 1

Antibody:	anti-Lef1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

S7A ©

S7C ©

Specimen S7A/C: embryonic day 14.5; Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Double labeled: green - Lef1; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS22: early nephron	Present		S7A , S7C

S7B ©

S7D ©

Specimen S7B/D: embryonic day 14.5; Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Mdm2^tm1.2Mep/Mdm2^tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Lef1; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS22: early nephron	Present		S7D , S7B	Lower expression in the nephron intermediates compared with the control. Lef1+, dsRed+ nascent nephrons were seen.

Specimen not shown: embryonic day 13.5; Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)

Structure	Level	Pattern	Image	Note
TS21: early nephron	Present

Specimen not shown: embryonic day 13.5; Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Mdm2^tm1.2Mep/Mdm2^tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: early nephron	Present			Lower expression in the nephron intermediates compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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