Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:192507
Kim JY, et al., Different expression patterns of Phactr family members in normal and injured mouse brain. Neuroscience. 2012 Sep 27;221:37-46
|
Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7469620
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Gene symbol:
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Phactr4
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Gene name:
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phosphatase and actin regulator 4
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Probe:
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Phactr4 probe
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Probe preparation:
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Antisense labelled with biotin RNA
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Visualized with:
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Fluorescence
|
|
Results
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Specimen
7A:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
Specimen
7A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
astrocyte |
Present |
Regionally restricted |
7A2
,
7A1
|
Expression in rostral migratory stream in Gfap-expressing astrocytes
|
Specimen
7B:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Dcx (assay MGI:7469628).
Specimen
7B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Dcx (assay MGI:7469628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
Present |
Regionally restricted |
7B2
,
7B1
|
Expression in rostral migratory stream not co-localized with Dcx.
|
Specimen
7C:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
Specimen
7C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
|
Specimen
7D:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Nes (assay MGI:7469622).
Specimen
7D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Nes (assay MGI:7469622).
|
Specimen
7E:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
Specimen
7E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: dentate gyrus |
astrocyte |
Present |
Regionally restricted |
7E1
,
7E2
,
7E
|
Expression in subgranular zone of dentate gyrus in Gfap-expressing astrocytes
|
Specimen
7F:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Nes (assay MGI:7469622).
Specimen
7F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Nes (assay MGI:7469622).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: dentate gyrus |
Present |
Regionally restricted |
7F1
,
7F
,
7F2
|
Expression in Nes-expressing cells.
|
Specimen
9A:
postnatal adult
(more )
Note: Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
Specimen
9A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Phactr4; red - Gfap (assay MGI:7469623).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
astrocyte |
Absent |
|
9A1
,
9A2
|
No expresson in Gfap-expressing astrocytes in cerebral cortex.
|
|