Antibody:	anti-Diaph2 (N-15): sc-10892
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Weak	Non-Uniform	S3A'' , S3A''' , S3A' , S3A	Expression is principally in the dorsal wall of the cochlear duct, which will develop into the organ of Corti.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Weak	Non-Uniform	S3B''' , S3B'' , S3B , S3B'''' , S3B'	Expression is principally in the dorsal wall of the cochlear duct, which will develop into the organ of Corti.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	cochlear outer hair cell	Strong		2B
TS26: cochlear duct epithelium		Present	Regionally restricted	2B	Expression is seen in Kolliker's organ.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present	Regionally restricted	2B'	Expression is seen in Kolliker's organ.
TS26: cochlear duct	Present	Regionally restricted	2B'	In the apical turn, similar to earlier stages, expression is localized in the luminal cells of the dorsal wall of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	cochlear outer hair cell	Strong		2B''
TS26: cochlear duct epithelium		Present	Regionally restricted	2B''	Expression is seen in Kolliker's organ.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	cochlear outer hair cell	Strong		2B'''
TS26: cochlear duct epithelium		Present	Regionally restricted	2B'''	Expression is seen in Kolliker's organ.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear outer hair cell	Strong		3A

Structure	Level	Pattern	Image	Note
TS27: cochlear duct	Present	Regionally restricted	3B	In the apical turn, similar to earlier stages, expression is localized in the luminal cells of the dorsal wall of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear outer hair cell	Strong		3F , 3C	Expression is seen in the stereocilia and at the base of the outer hair cells.
TS27: cochlear duct epithelium		Present	Regionally restricted	3C	Expression is seen in Kolliker's organ.
TS27: stria vascularis		Present		3E , 3C

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear outer hair cell	Strong		3D , 3G	Expression is seen in the stereocilia and at the base of the outer hair cells.
TS27: cochlear duct epithelium		Present	Regionally restricted	3D	Expression is seen in Kolliker's organ.
TS27: stria vascularis		Present		3D

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Weak		4A	Expression is at low levels in the sensory epithelium.
TS28: stria vascularis	Present		4A' , 4A
TS28: external spiral sulcus	Present	Regionally restricted	4A , 4A''	Expression is seen in the root cells and in the epithelial layer of Reissner's membrane.
TS28: scala media	Present	Regionally restricted	4A , 4A''	Expression is seen in the root cells and in the epithelial layer of Reissner's membrane.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Weak		4B	Expression is at low levels in the sensory epithelium.
TS28: stria vascularis	Present		4B , 4B'
TS28: scala media	Present	Regionally restricted	4B	Expression is seen in the root cells and in the epithelial layer of Reissner's membrane.
TS28: organ of Corti	Present		4B , 4B''

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Weak		S5_left

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Weak		S5_right