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Gene Expression Data
Assay Details
Assay
Reference: J:208435 Yi SH, et al., Foxa2 acts as a co-activator potentiating expression of the Nurr1-induced DA phenotype via epigenetic regulation. Development. 2014 Feb;141(4):761-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7468756
Gene symbol: Foxa2
Gene name: forkhead box A2
Antibody: anti-Foxa2 (M-20; sc-6554)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A/C: embryonic day 9.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 1A , 1C Expressed in the ventral region.
Specimen 1A inset: embryonic day 9.0 (more )
Note: Double labeled: green - Foxa2; red - Pcna (assay MGI:7468812).
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 1A inset Expressed in the ventral region, coexpressed with Pcna.
Specimen 1D/F: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 1D , 1F Expression was broader than that of Nr4a2, to the lateral portions.
TS20: midbrain ventricular layer Present 1D , 1F Weaker expression than in the mantle zone.
Specimen 1D inset: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Pcna (assay MGI:7468812).
Structure Level Pattern Image Note
TS20: midbrain Present 1D inset Some of the positive cells coexpressed Pcna.
Specimen 1G/I: embryonic day 15.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 1G , 1I Expression domain became narrower and was restricted to the domain of Nr4a2+ cells.
Specimen 1M: embryonic day 12.0 (more )
Note: Triple labeled: green - Foxa2; blue - Th (assay MGI:7468767); red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 1M
TS20: midbrain ventricular layer Present 1M
Specimen 5A: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Cell Type Level Pattern Image Note
TS20: midbrain mantle layer midbrain dopaminergic neuron Present 5A Foxa2 , 5A merge A granular distribution within the nucleus was shown, coexpressed with Nr4a2 (taken from Th+ cells in Fig 1M).
Specimen S1G: embryonic day 12.5 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S1G right , S1G left
TS20: midbrain ventricular layer Present S1G right , S1G left Weaker expression than in the mantle zone.
Specimen not shown: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: midbrain midbrain dopaminergic neuron Present Exclusively colocalized in Th+ dopaminergic neurons.
TS28: hippocampus hippocampal CA1-3 neuron Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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