Reporter:	GFP
Antibody:	anti-GFP
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		1A
TS26: cerebral cortex marginal zone	Present		1A
TS26: cerebral cortex subventricular zone	Present		1A
TS26: cortical plate	Present		1A
TS26: cortical subplate	Present		1A

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present	Single cells	1B	Some expressing cells incorporated BrdU.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present	Single cells	1C	Some expressing cells had condensed chromosomes, indicating they were undergoing mitosis.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present	Single cells	1D	Some expressing cells are proliferating; this is quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		S3A	The vast majority of expressing cells also express Map2 as quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		S3B	The vast majority of expressing cells also express Tubb3 as quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		S3C	The vast majority of expressing cells also express Dlx2 as quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		S3D	The vast majority of expressing cells also express Dcx as quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		S3E	The vast majority of expressing cells also express Ncam1 as quantified in Table S2.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present			All cells expressing the reporter also express the endogenous protein.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present	Clusters	S4B	Expressing cells were sometimes seen in clusters, which might be proliferating.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		S5A , S5B	Some expressing cells also express Ccnd1.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		S8B , S8A	A pair of reporter-expressing cells were observed; one cell also expressed Notch 3.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		S9A , S9B	A pair of reporter-expressing cells were observed; one cell also expressed Dll4.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present	Clusters	8A	The expressing cells appeared as cell chains/clusters; they do not co-express Rbfox3.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present	Clusters	8B	The expressing cells appeared as cell chains/clusters; these expressing cells may be surrounded by GFAP-positive processes.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present	Clusters	8C	The expressing cells appeared as cell chains/clusters; some of these expressing cells are proliferating.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Clusters	S11A	The expressing cells appeared as cell chains/clusters; they may also express Dcx.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		S12B , S12A	Expressing cells may be in close contact with Ascl1-expressing ones.