Probe:	Barhl2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1A	Expression is in two symmetric stripes.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	1B	Expression is in two symmetric stripes.
TS19: midbrain	Present	Regionally restricted	1B	Expression is in two symmetric stripes.
TS19: neural tube	Present	Regionally restricted	1B	Expression is in two symmetric stripes.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	1C	Expression is in the D1 dorsal sensory interneurons.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Regionally restricted	1D	Expression is in two small lateral columns between the alar and basal plates.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: piriform cortex	neuron	Present		1E
TS22: telencephalon		Present	Regionally restricted	1E	Expression is in the diagonal band.
TS22: epithalamus		Present		1E
TS22: preoptic area		Present		1E
TS22: retina		Present		1E
TS22: thalamus		Present	Regionally restricted	1E	Expression is in the intermediate thalamic neuroepithelia and ventral thalamus.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: piriform cortex	neuron	Present		1F
TS22: telencephalon		Present	Regionally restricted	1F	Expression is in the diagonal band.
TS22: epithalamus		Present		1F
TS22: preoptic area		Present		1F
TS22: thalamus		Present	Regionally restricted	1F	Expression is in the intermediate thalamic neuroepithelia and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		1G
TS22: mammillary area	Present		1G
TS22: midbrain tegmentum	Present		1G
TS22: preoptic area	Present		1G
TS22: superior colliculus	Present		1G
TS22: midbrain	Present	Regionally restricted	1G	Expression is in the periaqueductal gray area.

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		1H
TS22: midbrain tegmentum	Present		1H
TS22: superior colliculus	Present		1H
TS22: midbrain	Present	Regionally restricted	1H	Expression is in the periaqueductal gray area.
TS22: hindbrain	Present	Regionally restricted	1H	Expression is in the anterior extramural migratory stream and is absent from any of the precerebellar nuclei, including the pontine gray and reticulotegmental nuclei derived from the anterior extramural migratory stream.
TS22: medulla oblongata	Present	Regionally restricted	1H	Expression is in principal and spinal trigeminal nuclei.
TS22: cerebellum	Absent		1H

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	1I	Expression is in the anterior extramural migratory stream and is absent from any of the precerebellar nuclei, including the pontine gray and reticulotegmental nuclei derived from the anterior extramural migratory stream.
TS22: cerebellum	Absent		1I

Structure	Cell Type	Level	Pattern	Image	Note
TS27: nucleus of diagonal band		Present		1J
TS27: piriform cortex	neuron	Present		1J

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epithalamus		Present		1K
TS27: mammillary body		Present		1K
TS27: nucleus of diagonal band		Present		1K
TS27: paraventricular hypothalamic nucleus		Present		1K
TS27: preoptic area		Present		1K
TS27: piriform cortex	neuron	Present		1K
TS27: thalamus		Present	Regionally restricted	1K	Expression is in the intermediate thalamic neuroepithelia and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS27: epithalamus	Present		1L
TS27: paraventricular hypothalamic nucleus	Present		1L

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Present		1M
TS27: mammillary body	Present		1M
TS27: midbrain tegmentum	Present		1M
TS27: periaqueductal grey	Present		1M
TS27: superior colliculus	Present		1M

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		1N
TS27: inferior colliculus	Present		1N
TS27: midbrain tegmentum	Present		1N
TS27: periaqueductal grey	Present		1N
TS27: superior colliculus	Present		1N
TS27: trigeminal V spinal sensory nucleus	Present		1N
TS27: trigeminal nucleus	Present		1N
TS27: cerebellum	Present	Regionally restricted	1N	Expression is in the upper rhombic lip.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		1O
TS27: cerebellum	Present	Regionally restricted	1O	Expression is in the upper rhombic lip.

Structure	Level	Pattern	Image	Note
TS27: mammillary body	Present		1P
TS27: pontine nucleus	Absent		1P
TS27: reticular tegmental nucleus	Absent		1P

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted	2A	Expression is in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		2B
TS27: retina inner nuclear layer	Present		2B