Hsd3b1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:271281 Bowles J, et al., Retinoic Acid Antagonizes Testis Development in Mice. Cell Rep. 2018 Jul 31;24(5):1330-1341
Assay type:	RT-PCR
MGI Accession ID:	MGI:7466730
Gene symbol:	Hsd3b1
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Probe:	Mm01261921_mH
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Tbp.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Wt M	E14.5	TS22: testis	Present (a)	Not Specified	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Male
KO M	E14.5	TS22: gonad	Present (b)	Not Specified	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd	Male
Wt F	E14.5	TS22: ovary	Weak (a)	Not Specified	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Female
KO F	E14.5	TS22: ovary	Weak (c)	Not Specified	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd	Female

Notes:
(a) Expression levels are reduced in wild type XX gonads compared to XY ones.
(b) Expression levels in XY mutants are comparable to those seen in wild type.
(c) Expression levels in XX mutants are comparable to those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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