Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:271281 Bowles J, et al., Retinoic Acid Antagonizes Testis Development in Mice. Cell Rep. 2018 Jul 31;24(5):1330-1341
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7466615
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	Anti-DDX4/MVH (ab27591)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

3A XY Wt left ©

3A XY Wt left inset ©

Specimen 3A Wt AMH: embryonic day 13.5 (more

)
Note: Double labeled: red - Ddx4; green - Amh (assay MGI:7466639).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		3A XY Wt left , 3A XY Wt left inset

3A XY KO left ©

3A XY KO left inset ©

Specimen 3A KO AMH: embryonic day 13.5; Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd (more

)
Note: Double labeled: red - Ddx4; green - Amh (assay MGI:7466639).

Structure	Level	Pattern	Image	Note
TS21: germ cell of gonad	Present		3A XY KO left , 3A XY KO left inset

3A XY Wt right ©

3A XY Wt right inset ©

Specimen 3A Wt FOXL2: embryonic day 13.5 (more

)
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7466640).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		3A XY Wt right , 3A XY Wt right inset

3A XY KO right ©

3A XY KO right inset ©

Specimen 3A KO FOXL2: embryonic day 13.5; Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd (more

)
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7466640).

Structure	Level	Pattern	Image	Note
TS21: germ cell of gonad	Present		3A XY KO right , 3A XY KO right inset

4A XY Wt left ©

4A XY Wt left inset ©

Specimen 4A Wt SCC: embryonic day 13.5 (more

)
Note: Double labeled: red - Ddx4; green - Cyp11a1 (assay MGI:7466641).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		4A XY Wt left , 4A XY Wt left inset

4A XY KO left ©

4A XY KO left inset ©

Specimen 4A KO SCC: embryonic day 13.5; Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd (more

)
Note: Double labeled: red - Ddx4; green - Cyp11a1 (assay MGI:7466641).

Structure	Level	Pattern	Image	Note
TS21: germ cell of gonad	Present		4A XY KO left , 4A XY KO left inset

4A XY Wt right ©

4A XY Wt right inset ©

Specimen 4A Wt HSD3B: embryonic day 14.5 (more

)
Note: Double labeled: red - Ddx4; green - Hsd3b (assay MGI:7466642).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		4A XY Wt right inset , 4A XY Wt right

Specimen 4A KO HSD3B: embryonic day 14.5; Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd (more

)
Note: Double labeled: red - Ddx4; green - Hsd3b (assay MGI:7466642).

Structure	Level	Pattern	Image	Note
TS22: germ cell of gonad	Present		4A XY KO right inset , 4A XY KO right

5B XY Wt ©

Specimen 5B Wt: embryonic day 14.5 (more

)
Note: Double labeled: red - Ddx4; green - Sox9 (assay MGI:7466659).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		5B XY Wt inset , 5B XY Wt

5B XY KO ©

Specimen 5B KO: embryonic day 14.5; Cyp26b1^tm1Hmd/Cyp26b1^tm1Hmd (more

)
Note: Double labeled: red - Ddx4; green - Sox9 (assay MGI:7466659).

Structure	Level	Pattern	Image	Note
TS22: germ cell of gonad	Present		5B XY KO , 5B XY KO inset

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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