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Gene Expression Data
Assay Details
Assay
Reference: J:325355 Rong Y, et al., USP16-mediated histone H2A lysine-119 deubiquitination during oocyte maturation is a prerequisite for zygotic genome activation. Nucleic Acids Res. 2022 Jun 10;50(10):5599-5616
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7465105
Gene symbol: Rnf2
Gene name: ring finger protein 2
Antibody: anti-RNF2 (16031-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen S2 GV: postnatal adult (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: primary oocyte Present Regionally restricted S2_GV (merge) , S2_GV RNF2 Expresison primarily located in nucleus.
Specimen S2 GVBD: postnatal adult (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted S2_GVBD (merge) , S2_GVBD RNF2 Expression distributed in cytoplasm after nucleal envelope breakdown.
Specimen S2 MI: postnatal adult (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present S2_MI (merge) , S2_MI RNF2 Expression gradually accumulated during oocyte maturation.
Specimen S2 MII: postnatal adult (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present S2_MII (merge) , S2_MII RNF2 Expression gradually accumulated during oocyte maturation.
Specimen S2 Zygote: embryonic day 0.5 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted S2_Zygote RNF2 , S2_Zygote (merge) After fertilization, expression mainly localized to nucleus.
Specimen S2 2-cell: embryonic day 1.0 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S2_2-cell RNF2 , S2_2-cell (merge) After fertilization, expression mainly localized to nucleus.
Specimen S2 4-cell: embryonic day 2.0 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted S2_4-cell RNF2 , S2_4-cell (merge) After fertilization, expression mainly localized to nucleus.
Specimen S2 8-cell: embryonic day 2.5 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted S2_8-cell (merge) , S2_8-cell RNF2 After fertilization, expression mainly localized to nucleus.
Specimen S2 Morula: embryonic day 3.0 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted S2_Morula (merge) , S2_Morula RNF2 After fertilization, expression mainly localized to nucleus.
Specimen S2 Blastocyst: embryonic day 3.5 (more )
Note: Double labeled: red - Rnf2; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted S2_Blastocyst (merge) , S2_Blastocyst RNF2 After fertilization, expression mainly localized to nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory