Vim Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464766
Gene symbol:	Vim
Gene name:	vimentin

Antibody:	anti-vimentin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

7B WT ©

Specimen 7B WT: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens fiber	Strong		7B WT
TS25: lens epithelium	Weak		7B WT	very little positive staining

7B cKO ©

Specimen 7B cKO: embryonic day 17.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Present		7B cKO	Increased expression compared with the control.
TS25: lens fiber	Weak		7B cKO	Marked decreased expression compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24