Cdh1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464647
Gene symbol:	Cdh1
Gene name:	cadherin 1

Antibody:	anti-E-cadherin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

4A1 E13.5 WT ©

4A2 E13.5 WT ©

4A3 E13.5 WT ©

Specimen 4A E13.5 WT: embryonic day 13.5 (more

)

Structure	Level	Image	Note
TS21: lens anterior epithelium	Strong	4A1 E13.5 WT , 4A2 E13.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS21: lens equatorial epithelium	Strong	4A1 E13.5 WT , 4A3 E13.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS21: lens fiber	Absent	4A1 E13.5 WT

Specimen 4A E13.5 cKO: embryonic day 13.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS21: lens anterior epithelium	Present	4A1 E13.5 cKO , 4A2 E13.5 cKO	Trending toward decrease in epithelial width and reduced positive adheren junctions were seen.
TS21: lens equatorial epithelium	Present	4A1 E13.5 cKO , 4A3 E13.5 cKO	Trending toward decrease in epithelial width and reduced positive adheren junctions were seen.
TS21: lens fiber	Absent	4A1 E13.5 cKO

4A1 E15.5 WT ©

4A2 E15.5 WT ©

4A3 E15.5 WT ©

Specimen 4A E15.5 WT: embryonic day 15.5 (more

)

Structure	Level	Image	Note
TS23: lens anterior epithelium	Strong	4A1 E15.5 WT , 4A2 E15.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS23: lens equatorial epithelium	Strong	4A1 E15.5 WT , 4A3 E15.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS23: lens fiber	Absent	4A1 E15.5 WT

Specimen 4A E15.5 cKO: embryonic day 15.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS23: lens anterior epithelium	Present	4A2 E15.5 cKO , 4A1 E15.5 cKO	Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS23: lens equatorial epithelium	Present	4A3 E15.5 cKO , 4A1 E15.5 cKO	Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS23: lens fiber	Absent	4A1 E15.5 cKO

4A1 E17.5 WT ©

4A2 E17.5 WT ©

4A3 E17.5 WT ©

Specimen 4A E17.5 WT: embryonic day 17.5 (more

)

Structure	Level	Image	Note
TS25: lens anterior epithelium	Strong	4A2 E17.5 WT , 4A1 E17.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS25: lens equatorial epithelium	Strong	4A3 E17.5 WT , 4A1 E17.5 WT	A long columnar shape and intense staining in adherens junctions were seen.
TS25: lens fiber	Absent	4A1 E17.5 WT

Specimen 4A E17.5 cKO: embryonic day 17.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS25: lens anterior epithelium	Weak	4A2 E17.5 cKO , 4A1 E17.5 cKO	Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS25: lens equatorial epithelium	Weak	4A3 E17.5 cKO , 4A1 E17.5 cKO	Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS25: lens fiber	Absent	4A1 E17.5 cKO

Specimen not shown: embryonic day 15.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Present			Similar as in the wild-type.

Specimen not shown: embryonic day 17.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Present			Similar as in the wild-type.

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