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Gene Expression Data
Assay Details
Assay
Reference: J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464647
Gene symbol: Cdh1
Gene name: cadherin 1
Antibody: anti-E-cadherin
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A E13.5 WT: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: lens anterior epithelium Strong 4A1 E13.5 WT , 4A2 E13.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS21: lens equatorial epithelium Strong 4A1 E13.5 WT , 4A3 E13.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS21: lens fiber Absent 4A1 E13.5 WT

Specimen 4A E13.5 cKO: embryonic day 13.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: lens anterior epithelium Present 4A1 E13.5 cKO , 4A2 E13.5 cKO Trending toward decrease in epithelial width and reduced positive adheren junctions were seen.
TS21: lens equatorial epithelium Present 4A1 E13.5 cKO , 4A3 E13.5 cKO Trending toward decrease in epithelial width and reduced positive adheren junctions were seen.
TS21: lens fiber Absent 4A1 E13.5 cKO

Specimen 4A E15.5 WT: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: lens anterior epithelium Strong 4A1 E15.5 WT , 4A2 E15.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS23: lens equatorial epithelium Strong 4A1 E15.5 WT , 4A3 E15.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS23: lens fiber Absent 4A1 E15.5 WT

Specimen 4A E15.5 cKO: embryonic day 15.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lens anterior epithelium Present 4A2 E15.5 cKO , 4A1 E15.5 cKO Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS23: lens equatorial epithelium Present 4A3 E15.5 cKO , 4A1 E15.5 cKO Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS23: lens fiber Absent 4A1 E15.5 cKO

Specimen 4A E17.5 WT: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: lens anterior epithelium Strong 4A2 E17.5 WT , 4A1 E17.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS25: lens equatorial epithelium Strong 4A3 E17.5 WT , 4A1 E17.5 WT A long columnar shape and intense staining in adherens junctions were seen.
TS25: lens fiber Absent 4A1 E17.5 WT

Specimen 4A E17.5 cKO: embryonic day 17.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: lens anterior epithelium Weak 4A2 E17.5 cKO , 4A1 E17.5 cKO Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS25: lens equatorial epithelium Weak 4A3 E17.5 cKO , 4A1 E17.5 cKO Significant decrease (>40%) in epithelial width (apical to basal length) and alterations in epithelial cell shape. A dramatic and progressive loss was also observed in positive adheren junctions.
TS25: lens fiber Absent 4A1 E17.5 cKO

Specimen not shown: embryonic day 15.5; Tg(Pax6-cre,GFP)1Pgr/0 (more )
Structure Level Pattern Image Note
TS23: lens epithelium Present Similar as in the wild-type.

Specimen not shown: embryonic day 17.5; Tg(Pax6-cre,GFP)1Pgr/0 (more )
Structure Level Pattern Image Note
TS25: lens epithelium Present Similar as in the wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory