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Gene Expression Data
Assay Details
Assay
Reference: J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type: Western blot
MGI Accession ID: MGI:7464644
Gene symbol: Rap1a
Gene name: RAS-related protein 1a
Antibody: Anti-Rap1
Detection system: horseradish peroxidase
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Image: 2A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Genetic BackgroundMutant Allele(s)Sex Note
WT1 E17.5 TS25: lens Present C57BL/6 Not Specified
WT2 E17.5 TS25: lens Present C57BL/6 Not Specified
WT3 E17.5 TS25: lens Present C57BL/6 Not Specified
cKO1 E17.5 TS25: lens Trace involves: 129S/SvEv * C57BL/6 * FVB Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0Not Specified Conditional mutant.
cKO2 E17.5 TS25: lens Trace involves: 129S/SvEv * C57BL/6 * FVB Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0Not Specified Conditional mutant.
cKO3 E17.5 TS25: lens Trace involves: 129S/SvEv * C57BL/6 * FVB Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Pax6-cre,GFP)1Pgr/0Not Specified Conditional mutant.
Notes:
(a) Dramatic reduction (by ~90%) in the mutant compared with the control (summarized in Fig. 2B).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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