Jun Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203791 Kuracha MR, et al., Spry1 and Spry2 are necessary for eyelid closure. Dev Biol. 2013 Nov 15;383(2):227-38
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464171
Gene symbol:	Jun
Gene name:	jun proto-oncogene

Antibody:	anti-p-c-jun
Assay notes:	The antibody used recognizes the phosphorylated form of the protein.

Results

5A ©

5A' ©

Specimen 5A/A': embryonic day 14.5 (more

)
Note: upper eyelid

Structure	Cell Type	Level	Pattern	Image	Note
TS22: upper eyelid	peridermal cell	Present		5A' , 5A
TS22: conjunctiva epithelium	conjunctival epithelial cell	Present		5A

5B ©

5B' ©

Specimen 5B/B': embryonic day 14.5; Spry1^tm1Jdli/Spry1^tm1Jdli, Spry2^tm1Mrt/Spry2^tm1Mrt, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant. upper eyelid

Structure	Cell Type	Level	Pattern	Image	Note
TS22: upper eyelid	peridermal cell	Present		5B , 5B'	Reduced expression in the peridermal cells at the leading edge.
TS22: conjunctiva epithelium	conjunctival epithelial cell	Present		5B	Similar expression in the palpebral and bulbar conjunctival cells as in the control.

5C ©

5C' ©

Specimen 5C/C': embryonic day 14.5 (more

)
Note: lower eyelid

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lower eyelid	peridermal cell	Present		5C' , 5C
TS22: conjunctiva epithelium	conjunctival epithelial cell	Present		5C

5D ©

5D' ©

Specimen 5D/D': embryonic day 14.5; Spry1^tm1Jdli/Spry1^tm1Jdli, Spry2^tm1Mrt/Spry2^tm1Mrt, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant. lower eyelid

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lower eyelid	peridermal cell	Present		5D , 5D'	Reduced expression in the peridermal cells at the leading edge.
TS22: conjunctiva epithelium	conjunctival epithelial cell	Present		5D	Similar expression in the palpebral and bulbar conjunctival cells as in the control.

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