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Gene Expression Data
Assay Details
Assay
Reference: J:203791 Kuracha MR, et al., Spry1 and Spry2 are necessary for eyelid closure. Dev Biol. 2013 Nov 15;383(2):227-38
Assay type: RNA in situ
MGI Accession ID: MGI:7464151
Gene symbol: Spry2
Gene name: sprouty RTK signaling antagonist 2
Probe: Spry2 cDNA
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 1B: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: eye epithelium conjunctival epithelial cell Present 1B Palpebral and forniceal epithelial cells showed stronger expression than bulbar epithelial cells.
TS20: eye mesenchyme Present Regionally restricted 1B Expressed in mesenchymal cells adjacent to the conjunctival epithelia.

Specimen 1E: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: conjunctiva epithelium conjunctival epithelial cell Present 1E Palpebral and forniceal epithelial cells showed stronger expression than bulbar epithelial cells.
TS22: eyelid epidermal cell Present Regionally restricted 1E Expressed in the palpebral epidermal cells.
TS22: eyelid mesenchyme Present 1E Expression was broader.

Specimen 1H/H': embryonic day 15.5 (more )
Structure Cell Type Level Pattern Image Note
TS23: conjunctiva epithelium conjunctival epithelial cell Present 1H Palpebral and forniceal epithelial cells showed stronger expression than bulbar epithelial cells.
TS23: eyelid epidermal cell Present Regionally restricted 1H Expressed in the palpebral epidermal cells.
TS23: eyelid mesenchyme Present 1H' , 1H Expression was broader.
TS23: periderm peridermal cell Present Regionally restricted 1H' Expressed in peridermal cells at the leading edge of the eyelid.

Specimen 1L: embryonic day 14.5; Spry1tm1Jdli/Spry1tm1Jdli, Spry2tm1Mrt/Spry2tm1Mrt, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: conjunctiva epithelium conjunctival epithelial cell Trace 1L Near complete loss of expression was seen.
TS22: eyelid mesenchyme Present Regionally restricted 1L Increased expression in the mesenchymal cells adjacent to the bulbar and palpebral conjunctival epithelia compared with the control.
TS22: eyelid epidermal cell Trace Regionally restricted 1L Substantially reduced expression in the eyelid epidermal cells.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory