Gene Expression Data
Assay Details
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Assay
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Reference:
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J:203791
Kuracha MR, et al., Spry1 and Spry2 are necessary for eyelid closure. Dev Biol. 2013 Nov 15;383(2):227-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7464148
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Gene symbol:
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Spry1
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Gene name:
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sprouty RTK signaling antagonist 1
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Probe:
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Spry1 cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
1D:
embryonic day 14.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
conjunctival epithelial cell |
Present |
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1D
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Palpebral and forniceal epithelial cells showed stronger expression than bulbar epithelial cells.
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| TS22: eyelid |
epidermal cell |
Present |
Regionally restricted |
1D
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Expressed in the palpebral epidermal cells.
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| TS22: eyelid mesenchyme |
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Present |
Regionally restricted |
1D
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Expression was more localized. Expression was stronger in the mesenchymal cells closer to the margin.
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Specimen
1G/G':
embryonic day 15.5
(more )
Specimen
1G/G':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: conjunctiva epithelium |
conjunctival epithelial cell |
Present |
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1G
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Palpebral and forniceal epithelial cells showed stronger expression than bulbar epithelial cells.
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| TS23: eyelid |
epidermal cell |
Present |
Regionally restricted |
1G
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Expressed in the palpebral epidermal cells.
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| TS23: eyelid mesenchyme |
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Present |
Regionally restricted |
1G'
,
1G
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Expression was more localized. Expression was stronger in the mesenchymal cells closer to the margin.
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| TS23: periderm |
peridermal cell |
Present |
Regionally restricted |
1G'
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Expressed in peridermal cells at the leading edge of the eyelid.
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Specimen
1K:
(close )
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
conjunctival epithelial cell |
Trace |
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1K
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Near complete loss of expression was seen.
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| TS22: conjunctival sac |
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Present |
Regionally restricted |
1K
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Increased expression in the bulbar mesenchymal cells compared with the control.
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| TS22: eyelid mesenchyme |
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Present |
Regionally restricted |
1K
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Increased expression in the mesenchymal cells adjacent to the bulbar and palpebral conjunctival epithelia compared with the control.
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| TS22: eyelid |
epidermal cell |
Trace |
Regionally restricted |
1K
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Substantially reduced expression in the eyelid epidermal cells.
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Analysis Tools