Arl13b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:331469 Lee H, et al., Tissue-specific requirement of sodium channel and clathrin linker 1 (Sclt1) for ciliogenesis during limb development. Front Cell Dev Biol. 2022;10:1058895
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7463898
Gene symbol:	Arl13b
Gene name:	ADP-ribosylation factor-like 13B

Antibody:	anti-ARL13B
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 1E Forelimb Sclt1^+/tm1a: embryonic day 11.5; Sclt1^{tm1a(EUCOMM)Hmgu}/Sclt1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1E Sclt1^+/tm1a Forelimb	Expression is in cilia that extend from the basal body.

Specimen 1E Forelimb Sclt1^tm1a: embryonic day 11.5; Sclt1^{tm1a(EUCOMM)Hmgu}/Sclt1^{tm1a(EUCOMM)Hmgu} (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		1E Sclt1^tm1a Forelimb	Expression in this mutant is the same as in wild-type.

Specimen 1E Hindlimb Sclt1^+/tm1a: embryonic day 11.5; Sclt1^{tm1a(EUCOMM)Hmgu}/Sclt1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	1E Sclt1^+/tm1a Hindlimb	Expression is in cilia that extend from the basal body.

Specimen 1E Hindlimb Sclt1^tm1a: embryonic day 11.5; Sclt1^{tm1a(EUCOMM)Hmgu}/Sclt1^{tm1a(EUCOMM)Hmgu} (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Weak		1E Sclt1^tm1a Hindlimb	There is about a 50% reduction in staining in this mutant compared to wild-type.

Specimen S4C Cep83^+/+ top: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	S4C Cep83^+/+ top	Expression is in cilia in developing neuroepithelial cells.

Specimen S4C Cep83^+/+ bottom: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	S4C Cep83^+/+ bottom	Expression is in cilia in developing neuroepithelial cells.

Specimen S4C Cep83^KO top: embryonic day 9.5; Cep83^{tm1(KOMP)Vlcg}/Cep83^{tm1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak	Regionally restricted	S4C Cep83^KO top	Expression is in cilia in developing neuroepithelial cells.

Specimen S4C Cep83^KO bottom: embryonic day 9.5; Cep83^{tm1(KOMP)Vlcg}/Cep83^{tm1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak	Regionally restricted	S4C Cep83^KO bottom	Expression is in cilia in developing neuroepithelial cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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