Tg(Rr28-Igf2)#Ddp
Transgene Detail
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| Symbol: |
Tg(Rr28-Igf2)#Ddp |
| Name: |
transgene insertion, Dimitrina D Pravtcheva |
| MGI ID: |
MGI:3573695 |
| Synonyms: |
H19eIgfMlu, Igf2 |
| Transgene: |
Tg(Rr28-Igf2)#Ddp Location: unknown
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| Alliance: |
Tg(Rr28-Igf2)#Ddp page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Rr28-Igf2)#Ddp expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Igf2 (MGI:96434) |
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Mutation details: This transgene contains a 17 kb genomic fragment containing the entire coding region of a partially methylated mouse Igf2 under control of its own promoter. A small 5' fragment of the noncoding region of exon 1 was left out of this construct. The MluI restriction site at the 3' end of the nontranslated portion of exon 6 was abolished to distinguish the transgene from the endogenous Igf2 gene. A 7 kb genomic fragment containing the mouse Igf2/H19 enhancer sequences (Rr28) from downstream of H19 was cloned upstream of the Igf2 gene. RT-PCR analysis showed transgene expression equal to or slightly higher than the endogenous Igf2 protein in the liver, kidney, lung, heart, and mammary tissue.
(J:90325)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:90325 Wise TL, et al., Perinatal lethality in H19 enhancers-Igf2 transgenic mice. Mol Reprod Dev. 1997 Oct;48(2):194-207 |
| All: |
2 reference(s) |
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Analysis Tools
