Antibody:	Anti-Tgfbr3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Absent		1A
TS20: palatal shelf mesenchyme	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Weak

Structure	Cell Type	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	epithelial cell	Present	Regionally restricted	1B	Expression was localized exclusively to the medial edge epithelium.
TS22: palatal shelf mesenchyme		Absent		1B

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	1C	Expression was mainly localized to the medial edge epithelium.
TS22: nasal epithelium	Present	Regionally restricted	1C	Expression was localized adjacent to the midline.
TS22: oral epithelium	Present	Regionally restricted	1C	Expression was localized adjacent to the midline.
TS22: palatal shelf mesenchyme	Absent		1C

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	1D	Expression at the midline was discontinuous.
TS22: nasal epithelium	Present	Regionally restricted	1D	Intense staining was concentrated in the oral and nasal epithelial triangle areas.
TS22: oral epithelium	Present	Regionally restricted	1D	Intense staining was concentrated in the oral and nasal epithelial triangle areas.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Present	Regionally restricted	1E	Immunostaining was not found in the palatal mesenchyme except in transformed medial edge epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	1F	Expression at the midline was discontinuous.
TS22: nasal epithelium	Present	Regionally restricted	1F	Intense staining was concentrated in the oral and nasal epithelial triangle areas.
TS22: oral epithelium	Present	Regionally restricted	1F	Intense staining was concentrated in the oral and nasal epithelial triangle areas.