Antibody:	C592/7E
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	5A	Expression was found in tissues of ectodermal and mesodermal origin.
TS15: mesenchyme derived from somatopleure	Present		5A
TS15: mesenchyme derived from splanchnopleure	Present		5A

Structure	Cell Type	Level	Pattern	Image	Note
TS15: embryo		Present	Regionally restricted	5B	Expression was found in tissues of ectodermal and mesodermal origin.
TS15: notochord		Absent		5B
TS15: blood vessel	endothelial cell	Absent		5B
TS15: intermediate mesenchyme		Present		5B
TS15: mesenchyme derived from somatopleure		Present		5B
TS15: mesenchyme derived from splanchnopleure		Present		5B
TS15: hindgut mesenchyme		Present		5B
TS15: hindgut epithelium		Absent		5B	Expression was absent from the endodermal epithelium.

Structure	Level	Pattern	Image	Note
TS17: embryo mesoderm	Present		5C	Mesodermal expression remained constant in E10.5.
TS17: hindgut epithelium	Present		5C	Expression extended from the hindgut mesoderm into the epithelium.
TS17: hindgut mesenchyme	Present		5C	Expression extended from the hindgut mesoderm into the epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: embryo		Present	Regionally restricted	6A	Expression was found in tissues of ectodermal and mesodermal origin.
TS15: migrating neural crest	migratory neural crest cell	Present		6A

Structure	Cell Type	Level	Pattern	Image	Note
TS17: neural tube	motor neuron	Strong	Regionally restricted	6B	Expression was strong in differentiating motor neurons in the ventral horns.
TS17: neural tube	interneuron	Strong	Regionally restricted	6B	Expression was strong in a population of interneurons.
TS17: neural tube floor plate		Weak		6B
TS17: neural tube roof plate		Weak		6B
TS17: neural tube		Weak	Regionally restricted	6B	Expression in the ependymal layer was weak.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	6C	Expression was detected in a more anterior location up to the level of the 4th prevertebra. No expression was found in the ventral horns or ependymal layer at this axial level.
TS19: neural tube mantle layer	Present	Regionally restricted	6C	Expression was detected in a more anterior location up to the level of the 4th prevertebra. In this region was limited to a population in the mediolateral region of the mantle layer.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	6D	The posterior expression domain up to prevertebra 5 was consistent with a continuation of the pattern observed at E10.5. Expression was predominant in the ventral horns. Low levels of expression were found in the ependymal layer with an intensity gradient from ventral to dorsal.
TS19: neural tube mantle layer	Present	Regionally restricted	6D	Expression expanded concomitantly in the ventral and mediolateral region of the mantle layer.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6E	In proximity to the anterior boundary of Hoxc8 expression in the spinal cord, expression was in two stripes. A vertical band of cells expressing Hoxc8 extended along the ependymal layer in a dorsoventral orientation. A second, wedge-shaped group of cells was found in a medial to lateral orientation across the mantle layer.
TS20: dorsal root ganglion	Absent		6E	At this level of the spinal cord, the dorsal root ganglion was not stained.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6F	Expression gradually returned to the ventral horns towards more posterior levels.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6G	Expression was fully reconstituted at the level of the 7th prevertebra and persisted through the level of the 10th prevertebra.
TS20: dorsal root ganglion	Present	Regionally restricted	6G	At this level of the spinal cord, expression was also present in a subset of cells in dorsal root ganglia.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: thoracic dorsal root ganglion		Present	Regionally restricted	7A , 7B	Expression in the dorsal root ganglion coincided with the level of strong expression in the spinal cord.
TS20: sympathetic ganglion	neuron	Present	Regionally restricted	7A , 7B	Expression within the sympathetic chain was restricted to the three to four anterior-most, upper thoracic ganglia. Co-labeling with Map2 indicated the staining was associated with differentiating neurons.
TS20: spinal cord	neuron	Strong	Regionally restricted	7B , 7A	Expression was strong in the ventral horn of the spinal cord. Co-labeling with Map2 indicated the staining was associated with differentiating neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: sympathetic ganglion	neuron	Present	Regionally restricted	7C	Expression was detected in differentiating neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	spinal cord motor neuron	Present	Regionally restricted	7E	Coexpression of Hoxc8 and Prph was found in a small subset of cells in the spinal cord at the outer border of the mantle layer. These cells are likely to be presynaptic motor neurons. Other sets of motor neurons expressed Hoxc8 alone.

Structure	Level	Pattern	Image	Note
TS20: thoracic dorsal root ganglion	Present		7F

Structure	Cell Type	Level	Pattern	Image	Note
TS20: thoracic dorsal root ganglion	neuron	Present	Regionally restricted	7G	Colabeling of Hoxc8 and Prph was detected in differentiating neurons.