Antibody:	anti-TRPV1
Assay notes:	Adult eyes were fixed in 0.75% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: lens	Strong		4A	Expression extends from the anterior to posterior poles and from the equatorial epithelium to the lens core.
TS28: lens equatorial epithelium	Present		4A

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5D , 5B	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5D , 5B	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5D , 5B	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5F , 5H	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5F , 5H	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5F , 5H	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5L , 5J	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5L , 5J	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5L , 5J	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	6G , 6B	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	6G , 6B	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	6G , 6B	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	6H , 6C	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	6H , 6C	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	6H , 6C	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	6D , 6I	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	6D , 6I	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	6D , 6I	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	6J , 6E	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	6J , 6E	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	6J , 6E	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	6F , 6K	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	6F , 6K	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	6F , 6K	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		6R , 6M	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		6R , 6M	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		6R , 6M	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		6S , 6N	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		6S , 6N	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		6S , 6N	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		6O , 6T	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		6O , 6T	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		6O , 6T	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		6U , 6P	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		6U , 6P	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		6U , 6P	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		6Q , 6V	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		6Q , 6V	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		6Q , 6V	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS16: lens primordium	Strong		8A	Expression is at the membranes.

Structure	Level	Pattern	Image	Note
TS18: lens vesicle	Present		8C	Expression is at the membranes.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8F	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8F	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8H	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8H	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8J	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8J	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.