Lgr5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:334516 Reichmuth J, et al., Manipulating the murine Lgr5 locus using a rapid, efficient and flexible CRISPR/Cas9 pipeline. bioRxiv. 2020;
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7460276
Gene symbol:	Lgr5
Gene name:	leucine rich repeat containing G protein coupled receptor 5

Reporter:	RFP
Antibody:	anti-RFP (600-401-379)
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Results

Specimen 2a - hom: postnatal adult; Lgr5^{em1(cre/ERT2)Kba}/Lgr5^{em1(cre/ERT2)Kba} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	intestinal crypt stem cell of small intestine	Present		2a top	Expression is seen at the bottom of the crypt, where the intestinal epithelial stem cells are located. The signal is stronger in homs than hets.

Specimen 2a - het: postnatal adult; Lgr5^{em1(cre/ERT2)Kba}/Lgr5⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	intestinal crypt stem cell of small intestine	Present		2a middle	Expression is seen at the bottom of the crypt, where the intestinal epithelial stem cells are located. The signal is stronger in homs than hets.

Specimen 2c - hom: postnatal adult; Lgr5^{em1(cre/ERT2)Kba}/Lgr5^{em1(cre/ERT2)Kba} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: large intestine crypt of lieberkuhn	intestinal crypt stem cell of large intestine	Present		2c top	In the colon expression is seen at the bottom of the crypt, where the intestinal epithelial stem cells are located. The signal is stronger in homs than hets.

Specimen 2c - het: postnatal adult; Lgr5^{em1(cre/ERT2)Kba}/Lgr5⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: large intestine crypt of lieberkuhn	intestinal crypt stem cell of large intestine	Present		2c middle	In the colon expression is seen at the bottom of the crypt, where the intestinal epithelial stem cells are located. The signal is stronger in homs than hets.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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