Gene Expression Data
Assay Details
Assay
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Reference:
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J:52896
Masuda H, et al., Expression and localization of collagen-binding stress protein Hsp47 in mouse embryo development: comparison with types I and II collagen. Cell Stress Chaperones. 1998 Dec;3(4):256-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7451361
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Gene symbol:
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Col1a1
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Gene name:
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collagen, type I, alpha 1
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Antibody:
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Anti-Collagen Type I
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated. See Col1a2 assay MGI:7460297.
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Results
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Specimen
2C:
embryonic day 18.5
(more )
Specimen
2C:
(close )
Genetic Background:
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BALB/c
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
2F:
embryonic day 18.5
(more )
Specimen
2F:
(close )
Genetic Background:
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BALB/c
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: humerus |
|
Present |
Regionally restricted |
2F
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Expression was detected in the bone matrix.
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TS26: humerus |
osteoblast |
Present |
Regionally restricted |
2F
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Expression was detected in osteoblasts surrounding calcified bone matrix.
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Specimen
3B:
embryonic day 15.5
(more )
Specimen
3B:
(close )
Genetic Background:
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BALB/c
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: maxilla |
osteoblast |
Present |
Regionally restricted |
3B
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Expression was detected in osteoblasts in the intramembranous ossification of the maxilla.
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
Genetic Background:
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BALB/c
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Present |
|
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Expression was detected in the heart, especially in cushion tissue.
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Specimen
not shown E14.5:
embryonic day 14.5
(more )
Specimen
not shown E14.5:
(close )
Genetic Background:
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BALB/c
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: heart |
Present |
|
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Expression was detected in the heart, especially in cushion tissue.
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Specimen
7C:
embryonic day 15.5
(more )
Specimen
7C:
(close )
Genetic Background:
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BALB/c
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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