Antibody:	Anti-Hsp47
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS26: humerus	Present		2A
TS26: scapula	Present		2A

Structure	Cell Type	Level	Pattern	Image	Note
TS26: scapula	chondrocyte	Present	Regionally restricted	2B	Staining was detected in chondrocytes from the reserve zone to the hypertrophic zone. Zones of degeneration were faintly stained.
TS26: perichondrium		Present		2B
TS26: periosteum		Present		2B

Structure	Cell Type	Level	Pattern	Image	Note
TS26: humerus	osteoblast	Present	Regionally restricted	2E	Expression was detected in osteoblasts surrounding calcified bone matrix.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Weak	Regionally restricted	2H	Expression was weak in the prechondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: maxilla	osteoblast	Present	Regionally restricted	3A	Expression was detected in osteoblasts in the intramembranous ossification of the maxilla.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: head mesenchyme	mesenchymal cell	Strong		4A

Structure	Level	Pattern	Image	Note
TS24: face skin	Strong	Regionally restricted	4B	Expression was detected in snout skin. The mesenchyme underlying the epidermis and surrounding the developmental hair follicle was strongly stained.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: back of trunk	myoblast	Present		4C

Structure	Cell Type	Level	Pattern	Image	Note
TS25: back musculature	fibroblast	Present	Single cells	4D	Completive muscle of the back was not stained except for a few cells such as fibroblasts.

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Absent		5A
TS19: urinary system mesenchyme	Weak	Regionally restricted	5A	Mesenchyme surrounding the ureteric bud was weakly labeled.

Structure	Level	Pattern	Image	Note
TS25: renal convoluted tubule	Present		5B
TS25: renal corpuscle	Present		5B
TS25: Bowman's capsule of mature renal corpuscle	Strong		5B

Structure	Cell Type	Level	Pattern	Image	Note
TS25: mesenchyme of metanephros	mesenchymal cell	Present	Regionally restricted	5C	Expression was detected in mesenchymal cells surrounding the tubules.

Structure	Level	Pattern	Image	Note
TS17: heart	Present			Expression was detected in the heart, especially in cushion tissue.

Structure	Level	Pattern	Image	Note
TS22: heart	Present			Expression was detected in the heart, especially in cushion tissue.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: stomach mesenchyme	mesenchymal cell	Weak		6A
TS22: stomach epithelium		Absent		6A	Stomach epithelium derived from endoderm was not stained.

Structure	Level	Pattern	Image	Note
TS25: stomach glandular region muscularis mucosa	Present		6B
TS25: stomach glandular region submucosa	Present		6B

Structure	Level	Pattern	Image	Note
TS18: liver parenchyma	Absent		6C

Structure	Cell Type	Level	Pattern	Image	Note
TS20: midbrain vascular element	endothelial cell	Present		7A

Structure	Cell Type	Level	Pattern	Image	Note
TS23: choroid plexus epithelium	ependymal cell	Strong		7B
TS23: choroid plexus stroma	mesenchymal cell	Strong		7B

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		7D
TS17: notochord	Present		7D
TS17: neural tube	Present	Regionally restricted	7D	Expression was detected in the ventral area.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: brain ventricle	ependymal cell	Present	Regionally restricted	7E	Expression was detected in ependymal cells and migrating cells in the ventral area of the ventricle.