Antibody:	anti-Osteoadherin/OSAD (AF3308)
Detection system:	Secondary antibody coupled to other - see notes
Assay notes:	Primary antibody was detected with rabbit anti-goat antibody conjugated with 10nm gold particles.

Structure	Level	Pattern	Image	Note
TS23: tooth	Present	Regionally restricted	3A	Expressed in the highly active predentin.

Structure	Level	Pattern	Image	Note
TS27: predentin	Present	Graded	3B	The number of gold-particles detected increased with development. Expressed in a gradient from the proximal and central regions to the most distal at the mineralization front.

Structure	Level	Pattern	Image	Note
TS28: predentin	Present		3C	The elevated levels of expression were maintained. Localization close to collagen fibers were very clearly observed.

Structure	Level	Pattern	Image	Note
TS28: predentin	Present		3D	The elevated levels of expression were maintained.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: molar dental pulp	odontoblast	Trace		S5A	Few gold-labeled particles were detected.

Structure	Level	Pattern	Image	Note
TS28: predentin	Present		S5B	Increased expression in the highly active predentin.

Structure	Level	Pattern	Image	Note
TS28: dentin	Present		S5C	To some extent in the dentin layer.

Structure	Level	Pattern	Image	Note
TS28: enamel	Trace		S5D	Few gold-labeled particles were detected.