Antibody:	Biglycan antibody (LF-159)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: predentin	Very strong	Regionally restricted	2B	Expressed in the predentin but more closely located to the odontoblastic cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: dental sac		Absent		S2A
TS23: dental papilla	preodontoblast	Absent		S2A
TS23: enamel organ	preameloblast	Absent		S2A

Structure	Cell Type	Level	Pattern	Image	Note
TS25: dental papilla	preodontoblast	Absent		S2B
TS25: jaw		Weak	Regionally restricted	S2B	Expressed in the alveolar bone.
TS25: enamel organ	preameloblast	Absent		S2B
TS25: predentin		Absent		S2B

Structure	Cell Type	Level	Pattern	Image	Note
TS27: extracellular matrix		Very strong		S2C	Mostly restricted to the predentin but proximal to the odontoblast cell layer.
TS27: predentin		Very strong		S2C	Mostly restricted to the predentin but proximal to the odontoblast cell layer.
TS27: dentin		Strong	Regionally restricted	S2C
TS27: dental papilla	odontoblast	Absent		S2C
TS27: dental pulp		Weak		S2C
TS27: enamel organ	ameloblast	Absent		S2C
TS27: enamel		Absent		S2C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: extracellular matrix		Very strong		S2D
TS28: predentin		Very strong		S2D
TS28: dentin		Weak		S2D	More or less detectable with some variation.
TS28: dental papilla	odontoblast	Absent		S2D
TS28: enamel		Absent		S2D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: extracellular matrix		Strong		S2E
TS28: predentin		Strong		S2E
TS28: dentin		Weak		S2E	More or less detectable with some variation.
TS28: dental papilla	odontoblast	Absent		S2E

Structure	Level	Pattern	Image	Note
TS27: alveolar process	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS28: alveolar process		Absent
TS28: enamel organ	ameloblast	Absent

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Weak			More or less detectable with some variation.
TS28: molar dental pulp	Weak