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Gene Expression Data
Assay Details
Assay
Reference: J:332882 Li N, et al., CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos. Stem Cell Reports. 2023 Jan 10;18(1):81-96
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7451123
Gene symbol: Cpsf6
Gene name: cleavage and polyadenylation specific factor 6
Antibody: anti-CPSF6 [EPR12898] (ab175237)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4D Zygote: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 4D Zygote CPSF6 , 4D Zygote CPSF6/DAPI Expression both in nucleus and in cytoplasm.
Specimen 4D 2-cell: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 4D 2-cell CPSF6 , 4D 2-cell CPSF6/DAPI Expression both in nucleus and in cytoplasm. Nuclear expression became more detectable at 2-cell stage.
Specimen 4D 4-cell: embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted 4D 4-cell CPSF6 inset , 4D 4-cell CPSF6 , 4D 4-cell CPSF6/DAPI Nuclear expression remained throughout preimplantation. Granules of expression in cytoplasm observed at 4-cell stage.
Specimen 4D 8-cell: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 4D 8-cell CPSF6 , 4D 8-cell CPSF6 inset , 4D 8-cell CPSF6/DAPI Nuclear expression remained throughout preimplantation. Expression in cytoplasm peaked at 8-cell stage.
Specimen 4D Morula: embryonic day 3.0 (more )
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 4D Morula CPSF6/DAPI , 4D Morula CPSF6 inset , 4D Morula CPSF6 Nuclear expression remained throughout preimplantation. Expression in cytoplasm declined at morula stage.
Specimen 4D Blastocyst: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 4D Blastocyst CPSF6/DAPI , 4D Blastocyst CPSF6 Nuclear expression remained throughout preimplantation. Cytoplasmic expression disappeared in blastocyst.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory