Antibody:	claudin-6 Antibody (C-20): sc-17669
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Strong		2A	Expressed at not only the apical domain classically associated with tight junctions, but along the basolateral areas as well.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Strong		2B	Expressed at not only the apical domain classically associated with tight junctions, but along the basolateral areas as well.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		2C

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present		2E

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present		2F

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		3B , 3A	Coexpressed with Nkx2-1. Primarily localized to the plasma membrane.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		3D , 3E	Coexpressed with Nkx2-1. Primarily localized to the plasma membrane.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		4A , 4B	Despite weaker co-expression, membranous expression overlapped with Foxa2.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		4E , 4D	Highly consistent with the expression of Cldn6.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		4G , 4H	Coexpressed with Sox2.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		4K , 4J	Coexpressed with Sox9.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present			Reduced expression and coexpressed with Nkx2-1.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present			Coexpressed with Sox2 and Sox9.