Probe:	Nwd2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: habenula	Strong		4C , 4B
TS22: organ system	Present	Regionally restricted	4B	Only the habenula showed expression. Other organs and non-neural tissues showed no detectable expression.

Structure	Level	Pattern	Image	Note
TS22: habenula	Strong		4G , 4F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		4J , 4K	Most neurons were labeled.
TS28: lateral habenular nucleus	neuron	Present	Regionally restricted	4K , 4J	A small number of neurons were labeled.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		4N	Most neurons were labeled.
TS28: lateral habenular nucleus	neuron	Present	Regionally restricted	4N	A small number of neurons were labeled.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Absent		7B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak		7C

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Trace		7D

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Weak		7E

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Weak		7F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA3	hippocampal pyramidal neuron	Weak		7G
TS28: dentate gyrus	dentate gyrus neuron	Weak	Regionally restricted	7G	Expression was detected in mossy cells, which are excitatory hilar neurons sparsely distributed in the dentate gyrus.
TS28: dentate gyrus granule cell layer		Absent		7G
TS28: hippocampus CA1		Absent		7G
TS28: hippocampus CA2		Absent		7G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA3	hippocampal pyramidal neuron	Weak		7H
TS28: dentate gyrus	dentate gyrus neuron	Weak	Regionally restricted	7H	Expression was detected in mossy cells, which are excitatory hilar neurons sparsely distributed in the dentate gyrus.
TS28: dentate gyrus granule cell layer		Absent		7H
TS28: hippocampus CA1		Absent		7H
TS28: hippocampus CA2		Absent		7H

Structure	Cell Type	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	midbrain dopaminergic neuron	Present		8C , 8B
TS28: ventral tegmental area	midbrain dopaminergic neuron	Present		8C , 8B
TS28: substantia nigra pars reticulata	neuron	Present	Regionally restricted	8B , 8C	Some neurons were labeled.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Weak		8D

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		8F

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		8H

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	Purkinje cell	Present		8I

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	Purkinje cell	Present		8J

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		8L , 8K

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Absent		8N