Gjd3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:276168 Bhattacharyya S, et al., Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep. 2019 Feb 14;9(1):2106
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7449374
Gene symbol:	Gjd3
Gene name:	gap junction protein, delta 3

Antibody:	anti-Cx30.2 (40-7400)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

3c (i) Cx30.2 ©

3c (i) Merged ©

Specimen 3c - i: postnatal day 28; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Gjd3; magenta - Hcn4 (assay MGI:7449365); red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		3c (i) Merged , 3c (i) Cx30.2	Expression is seen in the distal node.

3c (ii) Cx30.2 ©

3c (ii) Merged ©

Specimen 3c - ii: postnatal day 28; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Gjd3; magenta - Hcn4 (assay MGI:7449365); red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		3c (ii) Merged , 3c (ii) Cx30.2	Expression in seen in the nodal extension.

S5(i) Cx30.2 ©

S5(i) Merged ©

Specimen S5 - i: embryonic day 12.5; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular canal	Present	Single cells	S5(i) Merged , S5(i) Cx30.2	Expression is seen in the vicinity of the presumptive atrioventricular node.

S8c upper Cx30.2 ©

S8c upper Merged ©

Specimen S8c - Wild-type: postnatal day 42 (more

)
Note: Triple labeled: green - Gjd3; magenta - Hcn4 (assay MGI:7449365); red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S8c upper Cx30.2 , S8c upper Merged

S8c lower Cx30.2 ©

S8c lower Merged ©

Specimen S8c - Het: postnatal day 42; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Gjd3; magenta - Hcn4 (assay MGI:7449365); red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S8c lower Cx30.2 , S8c lower Merged	Expression in the het is indistinguishable from that seen in wild type.

S10_E12.5 Cx30.2 ©

S10_E12.5 Merged ©

Specimen S10 - E12.5: embryonic day 12.5; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular canal	Present	Regionally restricted	S10_E12.5 Merged , S10_E12.5 Cx30.2	Expression is seen in the vicinity of the presumptive atrioventricular node.

S10_P1 Cx30.2 ©

S10_P1 Merged ©

Specimen S10 - P1: postnatal day 1; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS27: atrioventricular node	Present		S10_P1 Cx30.2 , S10_P1 Merged

S10_P4 Cx30.2 ©

Specimen S10 - P4: postnatal day 4; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S10_P4 Cx30.2 , S10_P4 Merged

Specimen S10 - P7: postnatal day 7; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S10_P7 Cx30.2 , S10_P7 Merged

Specimen S10 - P28: postnatal day 28; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S10_P28 Cx30.2 , S10_P28 Merged

Specimen S10 - P42: postnatal day 42; Gjd3^{tm1.1(cre/EGFP)Nmun}/Gjd3⁺, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: green - Gjd3; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS28: atrioventricular node	Present		S10_P42 Cx30.2 , S10_P42 Merged

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