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Gene Expression Data
Assay Details
Assay
Reference: J:297829 Li W, et al., Long non-coding RNA LncKdm2b regulates cortical neuronal differentiation by cis-activating Kdm2b. Protein Cell. 2020 Mar;11(3):161-186
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7449225
Gene symbol: Kdm2b
Gene name: lysine (K)-specific demethylase 2B
Reporter: GFP
Antibody: anti-GFP (#A11122)
Detection system: Secondary antibody
Results
Specimen 1D - left: embryonic day 16.5; Kdm2bem1(cre/ERT2)Bcgen/Kdm2b+ (more )
Note: Triple labeled: green - Kdm2b; red - Eomes (assay MGI:7451226); blue - Tubb3 (assay MGI:7451229).
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Present 1D left Expression extends farther basally than seen with in situs for the endogenous mRNA; this is probably because the EGFP protein is more stable than the transcript.
TS24: cerebral cortex subventricular zone Present 1D left Expression extends farther basally than seen with in situs for the endogenous mRNA; this is probably because the EGFP protein is more stable than the transcript.
Specimen 1D - right: embryonic day 16.5; Kdm2bem1(cre/ERT2)Bcgen/Kdm2b+ (more )
Note: Double labeled: green - Kdm2b; red - Unc5d (assay MGI:7451227).
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Present 1D right Expression extends farther basally than seen with in situs for the endogenous mRNA; this is probably because the EGFP protein is more stable than the transcript.
TS24: cerebral cortex subventricular zone Present 1D right Expression extends farther basally than seen with in situs for the endogenous mRNA; this is probably because the EGFP protein is more stable than the transcript.
Specimen S1R: embryonic day 14.5; Kdm2bem1(cre/ERT2)Bcgen/Kdm2b+ (more )
Note: Triple labeled: green - Kdm2b; red - Eomes (assay MGI:7451226); blue - Tubb3 (assay MGI:7451229).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present S1R EGFP , S1R Merge
TS22: cerebral cortex subventricular zone Present S1R EGFP , S1R Merge
Specimen S1S: embryonic day 14.5; Kdm2bem1(cre/ERT2)Bcgen/Kdm2b+ (more )
Note: Double labeled: green - Kdm2b; red - Unc5d (assay MGI:7451227).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present S1S EGFP , S1S Merged
TS22: cerebral cortex subventricular zone Present S1S EGFP , S1S Merged
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory