Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:225747 Jayewickreme CD, et al., Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol. 2015 Sep 1;405(1):21-32
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7448911
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-SMA-A
Detection system:	Secondary antibody coupled to fluorescein

Results

5A lower ©

5A middle ©

5A upper ©

Specimen 5A: embryonic day 17.5 (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: stomach wall	smooth muscle cell	Present		5A upper , 5A lower , 5A middle

5B lower ©

5B middle ©

5B upper ©

Specimen 5B: embryonic day 17.5 (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: intestine smooth muscle	enteric smooth muscle cell	Present		5B middle , 5B upper , 5B lower

5C lower ©

5C middle ©

5C upper ©

Specimen 5C: embryonic day 17.5; Gt(ROSA)26Sor^{tm1(Barx1)Shiv}/Gt(ROSA)26Sor⁺, Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: intestine smooth muscle	enteric smooth muscle cell	Present		5C middle , 5C lower , 5C upper	Significant smooth muscle expansion was seen (summarized in 5E).

5D lower ©

5D middle ©

5D upper ©

Specimen 5D: embryonic day 17.5; Barx1^tm1Shiv/Barx1^tm1Shiv (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: stomach wall	smooth muscle cell	Present		5D middle , 5D lower , 5D upper	Significant smooth muscle depletion was seen (summarized in 5E).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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