Antibody:	anti-NeuN
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1B CNS-ctr
TS28: corpus callosum	Present		1B CNS-ctr
TS28: fasciola cinereum	Present		1B CNS-ctr
TS28: hypothalamus	Present		1B CNS-ctr
TS28: medial habenular nucleus	Present		1B CNS-ctr
TS28: thalamus	Present		1B CNS-ctr

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1B CNS-del	Authors report ~20% reduction in thickness of cerebral cortex compared to littermate control.
TS28: corpus callosum	Present		1B CNS-del	Thickness of corpus callosum markedly reduced compared to control.
TS28: medial habenular nucleus	Present		1B CNS-del	Dramatic reduction in number of neurons compared to control.
TS28: fasciola cinereum	Present		1B CNS-del	Mild alteration of fasciola cinereum compared to control.
TS28: hypothalamus	Present		1B CNS-del	Size of thalamic and hypothalamic regions proportionately reduced compared to control.
TS28: thalamus	Present		1B CNS-del	Size of thalamic and hypothalamic regions proportionately reduced compared to control.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer I	Cajal-Retzius cell	Absent		1C CNS-ctr
TS28: cortical layer II	pyramidal neuron	Present		1C CNS-ctr	Expression in small pyramidal cells.
TS28: cortical layer III	pyramidal neuron	Present		1C CNS-ctr	Expression in small pyramidal cells.
TS28: cortical layer IV	cortical granule cell	Present		1C CNS-ctr
TS28: cortical layer V	pyramidal neuron	Present		1C CNS-ctr	Expression in large pyramidal cells.
TS28: cortical layer VI		Present		1C CNS-ctr	Expression in polymorphic cells.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Absent		1C CNS-del	Thickness of layer I similar to that of control.
TS28: cortical layer II	Present		1C CNS-del	Thickness of layer II/III reduced ~76% compared to control.
TS28: cortical layer III	Present		1C CNS-del	Thickness of layer II/III reduced ~76% compared to control.
TS28: cortical layer IV	Present		1C CNS-del	Thickness and cellularity decreased ~78% compared to control.
TS28: cortical layer V	Present		1C CNS-del	Thickness and cellularity decreased ~78% compared to control.
TS28: cortical layer VI	Present		1C CNS-del	Similar number of cells compared to control.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		1E NeuN CNS-ctr
TS28: hippocampus CA1	Present		1E NeuN CNS-ctr
TS28: hippocampus CA3	Present		1E NeuN CNS-ctr

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		1E NeuN CNS-del	Neurons in CA1, CA3 field and dentate gyrus less densely packed from P7 to P21.
TS28: hippocampus CA1	Present		1E NeuN CNS-del	Neurons in CA1, CA3 field and dentate gyrus less densely packed from P7 to P21.
TS28: hippocampus CA3	Present		1E NeuN CNS-del	Neurons in CA1, CA3 field and dentate gyrus less densely packed from P7 to P21.
TS28: dentate gyrus polymorphic layer	Present		1E NeuN CNS-del	Increased number of neurons compared to control.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		S1A NeuN CNS-ctr

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		S1A NeuN CNS-del	Authors report reduced cellularity and less defined laminar structure compared to control.

Structure	Level	Pattern	Image	Note
TS28: telencephalon subventricular zone	Present	Regionally restricted	S1B NeuN CNS-ctr	Expression in proliferating neuroprogenitors in SVZ of lateral ventricles.
TS28: rostral migratory stream	Absent		S1B NeuN CNS-ctr	No expression in immature neurons in rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: telencephalon subventricular zone	Present		S1B NeuN CNS-del	Proliferating neuroprogenitors in SVZ of lateral ventricles greatly reduced compared to control.
TS28: rostral migratory stream	Absent		S1B NeuN CNS-del	No expression in immature neurons in rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		S1C CNS-ctr

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		S1C CNS-del;Trp53+/+

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		S1C CNS-del;Trp53+/-	Authors report partial rescue of laminar structures

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb		Present		S1C CNS-del;Trp53-/-	Authors report partial rescue of laminar structures
TS28: main olfactory bulb granule cell layer	olfactory granule cell	Present		S1C CNS-del;Trp53-/-	Number of granule cells increased compared to control.