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Gene Expression Data
Assay Details
Assay
Reference: J:334115 Neirijnck Y, et al., Single-cell transcriptomic profiling redefines the origin and specification of early adrenogonadal progenitors. Cell Rep. 2023 Feb 28;42(3):112191
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7448480
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-Lef1 (#2230)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3B E9.0 LEF1: embryonic day 9.0 (more )
Note: Triple labeled: blue - Lef1; red - Gata4 (assay MGI:7448479); yellow - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS15: urogenital ridge Present 3B E9.0 LEF1 , 3B E9.0 MERGE (upper) , 3B E9.0 MERGE (lower)
Specimen 3B E10.5 LEF1: embryonic day 10.5 (more )
Note: Triple labeled: blue - Lef1; red - Gata4 (assay MGI:7448479); yellow - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS17: gonad primordium Present 3B E10.5 MERGE (lower) , 3B E10.5 LEF1 , 3B E10.5 MERGE (upper) Expression in gonadal progenitors,
TS17: adrenogonadal primordium Present Regionally restricted 3B E10.5 MERGE (upper) , 3B E10.5 LEF1 , 3B E10.5 MERGE (lower) No expression in adrenal progenitors.
Specimen 3B E11.5 LEF1: embryonic day 11.5 (more )
Note: Triple labeled: blue - Lef1; red - Gata4 (assay MGI:7448479); yellow - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS19: gonad Present 3B E11.5 MERGE (lower) , 3B E11.5 LEF1 , 3B E11.5 MERGE (upper) Expression in gonadal progenitors,
TS19: adrenal primordium Absent 3B E11.5 MERGE (lower) , 3B E11.5 LEF1 , 3B E11.5 MERGE (upper) No expression in adrenal progenitors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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