Gene Expression Data
Assay Details
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Assay
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Reference:
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J:255349
Rauch F, et al., Crispr-Cas9 engineered osteogenesis imperfecta type V leads to severe skeletal deformities and perinatal lethality in mice. Bone. 2018 Feb;107:131-142
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7447467
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Gene symbol:
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Ifitm5
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Gene name:
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interferon induced transmembrane protein 5
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 1% PFA, 0.25 mM ethylene glycol tetraacetic acid (EGTA), 0.2% (v/v) NP-40, 0.1 mM magnesium chloride (MgCl2), 0.2% glutaraldehyde.
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Results
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Specimen
7 WT/LacZ E14.5:
embryonic day 14.5; Ifitm5tm1Pmof/Ifitm5 +
(more  )
Specimen
7 WT/LacZ E14.5:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ifitm5tm1Pmof/Ifitm5+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 KI/LacZ E14.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ifitm5tm1Pmof/Ifitm5em1Pmof
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 WT/LacZ E18.5:
embryonic day 18.5; Ifitm5tm1Pmof/Ifitm5 +
(more )
Specimen
7 WT/LacZ E18.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ifitm5tm1Pmof/Ifitm5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 KI/LacZ E18.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ifitm5tm1Pmof/Ifitm5em1Pmof
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Analysis Tools