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Gene Expression Data
Assay Details
Assay
Reference: J:236440 You L, et al., The chromatin regulator Brpf1 regulates embryo development and cell proliferation. J Biol Chem. 2015 May 1;290(18):11349-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7447073
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubb3 (PRB-435P)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: Fixation was with 12% glacial acetic acid.
Results
Specimen 5B,C Control: embryonic day 9.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1+ (more )
Structure Cell Type Level Pattern Image Note
TS15: future hindbrain neuron Present Regionally restricted 5B Control , 5C Control w/DAPI , 5B Control w/DAPI , 5C Control In cephalic region, expression in post-mitotic neurons, concentrated at outer rim of neural tube.
Specimen 5B,C Brdf1 delta/delta: embryonic day 9.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1tm1c(EUCOMM)Wtsi, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS15: future hindbrain neuron Weak Regionally restricted 5B Brpf1 delta/delta w/DAPI , 5C Brpf1 delta/delta w/DAPI , 5B Brpf1 delta/delta , 5C Brpf1 delta/delta Fewer expressing neurons, some of which have migrated abnormally.
Specimen 5D,E Control: embryonic day 9.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1+ (more )
Structure Cell Type Level Pattern Image Note
TS15: future spinal cord neuron Present Regionally restricted 5D Control , 5E Control , 5D Control w/DAPI , 5E Control w/DAPI In trunk region, expression in post-mitotic neurons, concentrated at outer rim of neural tube.
Specimen 5D,E Brdf1 delta/delta: embryonic day 9.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1tm1c(EUCOMM)Wtsi, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS15: future spinal cord neuron Weak Regionally restricted 5E Brpf1 delta/delta , 5D Brpf1 delta/delta w/DAPI , 5D Brpf1 delta/delta , 5E Brpf1 delta/delta w/DAPI Fewer expressing neurons, some of which have migrated abnormally.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory