Mfsd2a In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278094 Pu W, et al., Genetic Targeting of Organ-Specific Blood Vessels. Circ Res. 2018 Jun 22;123(1):86-99
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7446568
Gene symbol:	Mfsd2a
Gene name:	MFSD2 lysolipid transporter A, lysophospholipid

Reporter:	ESR
Antibody:	anti-ESR (ab27595)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen Online VIIIA: postnatal adult; Mfsd2a^{em1(cre/ERT2)Bzsh}/Mfsd2a⁺ (more

)
Note: Double labeled: red - Mfsd2a reporter; green - Cdh5 (assay MGI:7451396).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain blood vessel	blood vessel endothelial cell	Present		Online VIIIA Merge , Online VIIIA ESR	Expression is seen in endothelial cells of the blood-brain barrier.

Specimen Online VIIIB: postnatal adult; Mfsd2a^{em1(cre/ERT2)Bzsh}/Mfsd2a⁺ (more

)
Note: Double labeled: red - Mfsd2a reporter; green - Rbfox3 (assay MGI:7451392).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	central nervous system neuron	Present		Online VIIIB ESR , Online VIIIB Merge

Specimen Online VIIIC: postnatal adult; Mfsd2a^{em1(cre/ERT2)Bzsh}/Mfsd2a⁺ (more

)
Note: Double labeled: red - Mfsd2a reporter; green - Hnf4a (assay MGI:7460336).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: liver	hepatocyte	Present		Online VIIIC ESR , Online VIIIC Merge

Specimen Online VIIID: postnatal adult; Mfsd2a^{em1(cre/ERT2)Bzsh}/Mfsd2a⁺ (more

)
Note: Double labeled: red - Mfsd2a reporter; green - Cdh1 (assay MGI:7460337).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: intestinal villus	epithelial cell of small intestine	Present		Online VIIID Merge , Online VIIID ESR

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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