Antibody:

Anti-Sox2 (Y-17; sc-17320)

Structure	Level	Pattern	Image	Note
TS13: trunk	Present		1Aii right , 1Aii center

Structure	Level	Pattern	Image	Note
TS13: trunk	Present		1Aiv center , 1Aiv right

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	1Avi center , 1Avi right	Expression was detected in the caudal lateral epiblast (CLE). Cells co-expressing Tbx6 were noted.
TS13: primitive streak	Present		1Avi center , 1Avi right	Cells co-expressing Tbx6 were noted.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present		1Aviii right , 1Aviii center

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	1B boxed area right , 1B boxed area center , 1B	Tbx6+Sox2+ cells were detected near the midline of the primitive streak/caudal lateral epiblast.
TS13: trunk	Present	Regionally restricted	1B boxed area right , 1B boxed area center , 1B	Tbx6+Sox2+ cells were detected near the midline of the primitive streak/caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	2_Top right , 2_Top center	Tbx6+Sox2+ neuromesoderm progenitors were detected in the chordo-neural hinge in the tail bud.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	3C Sox2 , 3C merge	Expression was detected in neuromesoderm progenitors.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	4B Tbx6YFP/+ merge left , 4B Tbx6YFP/+ merge right , 4B Tbx6YFP/+ Sox2	Co-expression of YFP with Sox2 was 28% in the heterozygote chordo-neural hinge.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	4B Tbx6YFP/YFP merge left , 4B Tbx6YFP/YFP merge right , 4B Tbx6YFP/YFP Sox2	A 74% overlap of YFP with Sox2 expression was detected in the mutant chordo-neural hinge.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 1	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 2	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 3	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 4	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 5	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6i Sox2 , S6i merge	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6ii Sox2 , S6ii merge	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6iii Sox2 , S6iii merge	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.