Antibody:

Anti-Tbx6

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present		1Aii left , 1Aii right

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present		1Aiv right , 1Aiv left
TS13: trunk	Present	Regionally restricted	1Aiv right , 1Aiv left	Expression was detected in the caudal lateral epiblast (CLE).

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present		1Avi right , 1Avi left
TS13: trunk	Present	Regionally restricted	1Avi right , 1Avi left	Expression was detected in the caudal lateral epiblast (CLE). Cells co-expressing Sox2 were noted.
TS13: primitive streak	Present		1Avi right , 1Avi left	Cells co-expressing Sox2 were noted.

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present		1Aviii left , 1Aviii right
TS13: trunk	Present	Regionally restricted	1Aviii right , 1Aviii left	Expression was detected in the caudal lateral epiblast (CLE).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	1B , 1B boxed area right , 1B boxed area left	Tbx6+Sox2+ cells were detected near the midline of the primitive streak/caudal lateral epiblast.
TS13: trunk	Present	Regionally restricted	1B , 1B boxed area right , 1B boxed area left	Tbx6+Sox2+ cells were detected near the midline of the primitive streak/caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	2_Top left , 2_Top right	Tbx6+Sox2+ neuromesoderm progenitors were detected in the chordo-neural hinge in the tail bud.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	2_Bottom left , 2_Bottom right	Expression was in neuromesoderm progenitors in the chordo-neural hinge in the tail bud.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	3C merge , 3C Tbx6	Expression was detected in neuromesoderm progenitors.

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present	Regionally restricted	S1_E8.5 embryo	Expression was detected in presomitic mesoderm in the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS19: tail	Present		S1_E11.5 tailbud

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 1	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 2	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 3	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 4	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	S2A 5	Tbx6+Sox2+ cells were detected near the midline of the caudal lateral epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6i Tbx6 , S6i merge	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6ii merge , S6ii Tbx6	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	S6iii Tbx6 , S6iii merge	Rare Tbx6+Sox2+ cells were detected in the node-primitive streak border.