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Gene Expression Data
Assay Details
Assay
Reference: J:276168 Bhattacharyya S, et al., Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep. 2019 Feb 14;9(1):2106
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7444443
Gene symbol: Gjd3
Gene name: gap junction protein, delta 3
Reporter: GFP
Antibody: anti-GFP (#A10262)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Tyramide signal amplification (TSA) was used to obtain a signal.
Results
Specimen 2b - iii: embryonic day 12.5; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Gjd3 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS20: atrioventricular canal Present Regionally restricted 2b E12.5 (iii) inset , 2b E12.5 (iii) GFP Expression is seen in the vicinity of the presumptive atrioventricular node.
Specimen S3 - i: postnatal day 4; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Gjd3 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present S3(i) Merged , S3(i) GFP
Specimen S4: postnatal day 7; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Gjd3 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present S4(i) Merged , S4(i) GFP
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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