Probe:	LR11 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak		4A

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Weak		4B	Expression in cerebral cortex layers II to VI.
TS28: cortical layer III	Weak		4B	Expression in cerebral cortex layers II to VI.
TS28: cortical layer IV	Weak		4B	Expression in cerebral cortex layers II to VI.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		4C
TS28: hippocampus	Present		4C

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Present	Regionally restricted	4D	Expression in dense deposits in neuron bodies of pyramidal layer.
TS28: hippocampus granule cell layer	Weak		4D	Lesser extent of expression in cerebellum granular layer.
TS28: dentate gyrus	Present		4D

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellar cortex	Purkinje cell	Present	Regionally restricted	4F	Expression in Purkinje cell bodies.

Structure	Level	Pattern	Image	Note
TS21: allocortex	Strong	Regionally restricted	6Bb	Abundant expression in hippocampal formation.
TS21: cerebral cortex	Strong		6Bb	Abundant expression in cortical neuroepithelia.

Structure	Level	Pattern	Image	Note
TS22: allocortex	Strong	Regionally restricted	6Bc	High levels of expression in hippocampal formation.
TS22: cerebral cortex	Strong		6Bc	Abundant expression in cortical neuroepithelia.

Structure	Level	Pattern	Image	Note
TS23: allocortex	Strong	Regionally restricted	6Bd	High levels of expression in hippocampal formation.
TS23: cerebral cortex	Strong		6Bd	High levels of expression in cortical neuroepithelia.

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Strong	Regionally restricted	6Be	High levels of expression in hippocampal formation.
TS24: cerebral cortex	Strong		6Be	High levels of expression in cortical neuroepithelia.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	7_Cerebral cortex week 1	Abundant expression in neural bodies of cerebral cortex at 1 and 2 weeks after birth.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	7_Cerebral cortex week 2	Abundant expression in neural bodies of cerebral cortex at 1 and 2 weeks after birth.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7_Cerebral cortex week 4	Expression decreased by 4 and 8 weeks.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7_Cerebral cortex week 8	Expression decreased by 4 and 8 weeks.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		7_Cerebral cortex week 12	Expression increased again in adult.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		7_ Hippocampus week 1	Pronounced expression at 1 week.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Present		7_ Hippocampus week 2	Expression decreased at 2 weeks.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Present		7_ Hippocampus week 4

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		7_ Hippocampus week 8	Expression increased from 8 weeks onward.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		7_ Hippocampus week 12	Expression increased from 8 weeks onward.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		7_ Dentate gyrus week 1	Pattern of expression similar to that in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7_ Dentate gyrus week 2	Pattern of expression similar to that in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7_ Dentate gyrus week 4	Pattern of expression similar to that in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		7_ Dentate gyrus week 8	Pattern of expression similar to that in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		7_ Dentate gyrus week 12	Pattern of expression similar to that in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		7_ Cerebellum week 2	High levels of expression at 2 weeks in both granular and Purkinje cell layers.
TS28: cerebellum granule cell layer	Strong		7_ Cerebellum week 2	High levels of expression at 2 weeks in both granular and Purkinje cell layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer		Present		7_ Cerebellum week 4	Decreased expression in granular cell layer.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		7_ Cerebellum week 4	High levels of expression maintained in Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer		Present		7_ Cerebellum week 8	Decreased expression in granular cell layer.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		7_ Cerebellum week 8	High levels of expression maintained in Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer		Present		7_ Cerebellum week 12	Decreased expression in granular cell layer.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		7_ Cerebellum week 12	High levels of expression maintained in Purkinje cells.