Probe:	MGC clone 67317
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Present	Regionally restricted	3O	Expression was detected in the endothelium.

Structure	Level	Pattern	Image	Note
TS22: atrioventricular valve	Present	Regionally restricted	3P	Expression was detected in the endothelium.

Structure	Level	Pattern	Image	Note
TS22: semilunar valve	Present	Regionally restricted	3Q	Expression was detected in the endothelium.

Structure	Level	Pattern	Image	Note
TS15: atrioventricular endocardial cushion	Present	Regionally restricted	4A' , 4A	Expression was detected in the cardiac endothelium.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Present	Regionally restricted	4B	Expression was detected in the tip cushion endothelium.

Structure	Level	Pattern	Image	Note
TS17: outflow tract endocardial cushion	Present	Regionally restricted	4C	Expression was detected in the tip cushion endothelium.

Structure	Level	Pattern	Image	Note
TS21: aortic valve	Present	Regionally restricted	4D	Expression was detected on both the ventricularis and fibrosa domains of the valve endothelium.

Structure	Level	Pattern	Image	Note
TS21: pulmonary valve	Present	Regionally restricted	4E	Expression was detected on both the ventricularis and fibrosa domains of the valve endothelium.

Structure	Level	Pattern	Image	Note
TS21: tricuspid valve	Present	Regionally restricted	4F	Expression was detected along the tip endothelium.

Structure	Level	Pattern	Image	Note
TS21: mitral valve	Present	Regionally restricted	4G	Expression was detected along the tip endothelium.

Structure	Level	Pattern	Image	Note
TS22: pulmonary valve	Present	Regionally restricted	4H	Expression was detected on both the ventricularis and fibrosa domains of the valve endothelium.

Structure	Level	Pattern	Image	Note
TS22: aortic valve	Present	Regionally restricted	4I	Expression was detected on both the ventricularis and fibrosa domains of the valve endothelium.

Structure	Level	Pattern	Image	Note
TS22: tricuspid valve	Present	Regionally restricted	4J	Expression became more restricted the tip region.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Present	Regionally restricted	4K	Expression became more restricted the tip region.

Structure	Level	Pattern	Image	Note
TS26: pulmonary valve leaflet	Present	Regionally restricted	4L	Expression was detected in the ventricularis endothelium of the valve leaflets.

Structure	Level	Pattern	Image	Note
TS26: aortic valve leaflet	Present	Regionally restricted	4M	Expression was detected in the ventricularis endothelium of the valve leaflets.

Structure	Level	Pattern	Image	Note
TS26: tricuspid valve	Present	Regionally restricted	4N	Expression was detected in the tip endothelium.

Structure	Level	Pattern	Image	Note
TS26: mitral valve	Present	Regionally restricted	4O	Expression was detected in the tip endothelium.

Structure	Level	Pattern	Image	Note
TS13: vascular system	Present	Regionally restricted	s4B' , s4B , s4B''	Expression was detected in branchial arch and intersomitic vessels.
TS13: heart	Absent		s4B' , s4B