Antibody:	anti-Phosphacan
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS21: lens	Very strong		1A
TS21: neural retina	Very strong		1A	Expression is in all regions of the retina particularly at the peripheral margin and the inner layer of the retina, which include the optic nerve fiber layer and the optic disc.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	1B	Expression is in a laminated pattern in the inner regions of the retina and is weak at the outer region adjacent to the pigment epithelium.

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	1C	Expression is in a laminated pattern in the inner regions of the retina and is weak at the outer region adjacent to the pigment epithelium.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Very strong	Regionally restricted	1D	Expression is at the optic fiber layer and a cell-rich layer within the inner parts of the retina.
TS23: retina	Weak	Regionally restricted	1D	Expression is in the outer margin of the retina, adjacent to the pigment epithelium.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Very strong	Regionally restricted	2A	Expression is in distinct domains in the optic pathway. Expression is in the glial-like profiles that radiate from the third ventricle and in an inverted V-shaped array in the caudal parts of the diencephalon.
TS21: optic stalk	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Very strong	Regionally restricted	2B	Expression is adjacent to the stalk labeling the profiles that extend from the third ventricle to the pial surface of the brain.
TS21: optic stalk	Absent		2B

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Very strong	Regionally restricted	2C	Expression is in the glial-like profiles that radiate from the third ventricle and in an inverted V-shaped array in the caudal parts of the diencephalon. Expression is absent in axon-like profiles in the chiasm.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Very strong	Regionally restricted	2I	Expression is in the glial-like profiles that radiate from the third ventricle and in an inverted V-shaped array in the caudal parts of the diencephalon.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2J	Expression is adjacent to the stalk labeling the profiles that extend from the third ventricle to the pial surface of the brain.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3A	Expression is in a strip of strongly labeled area, cut transversely in horizontal sections. In the optic pathway expression was weak in the stalk and the tract and the staining in the chiasm was reduced substantially in regions flanking both sides of the midline.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3B	Expression is in a strip of strongly labeled area, cut transversely in horizontal sections.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3C	In the optic pathway expression was weak in the stalk and the tract and the staining in the chiasm was reduced substantially in regions flanking both sides of the midline. Expression is in regions caudal to the chiasm, in a configuration mimicking the chiasmatic neurons.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	5A	Expression is in the lateral regions of the ventral diencephalon, marking some radial glial like profiles that extend from the third ventricle.
TS22: optic stalk	Weak		5A

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	5B	In this section cut across the mid-chiasm, expression was observed in the lateral regions and at the midline of the chiasm and the paramidline regions were weakly stained.

Structure	Level	Pattern	Image	Note
TS22: optic tract	Present	Regionally restricted	5C	Expression is in the deep but not the subpial regions of the tract.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Strong	Regionally restricted	6A	Expression is at the lateral region of the mid-chiasm. Expression was reduced in the medial regions of the chiasm, except the midline, where an expression-rich raphe was observed extending from the chiasmatic neurons in the caudal regions of the diencephalon.
TS23: optic stalk	Moderate		6A
TS23: optic tract	Moderate		6A

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Strong	Regionally restricted	6B	Expression is at the lateral region of the mid-chiasm. Expression was reduced in the medial regions of the chiasm, except the midline, where an expression-rich raphe was observed extending from the chiasmatic neurons in the caudal regions of the diencephalon.
TS23: optic stalk	Moderate		6B
TS23: optic tract	Moderate		6B