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Gene Expression Data
Assay Details
Assay
Reference: J:177404 Schneider M, et al., Cell-specific detection of microRNA expression during cardiomyogenesis by combined in situ hybridization and immunohistochemistry. J Mol Histol. 2011 Aug;42(4):289-99
Assay type: RNA in situ
MGI Accession ID: MGI:7442494
Gene symbol: Mir199a-1
Gene name: microRNA 199a-1
Probe: miR-199a
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Rhodamine
Assay notes: The mature microRNA is encoded at multiple sites in the genome.
Results
Specimen 3 miR-199a: embryonic day 14.5 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS22: heart Present Regionally restricted 3_miR-199a Expression was widely distributed within connective tissues of the heart.
TS22: dorsal aorta Present Regionally restricted 3_miR-199a Expression was localized to the aortic wall.
TS22: atrioventricular septal complex Present Regionally restricted 3_miR-199a Expression was detected in streaks of connective tissue in the atrioventricular junction.
Specimen 4c: postnatal day 98 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS28: artery Weak Regionally restricted 4c* , 4c Low expression was detected in vessel walls.
Specimen 4f: postnatal day 98 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS28: left atrium Ambiguous 4f* , 4f
Specimen 4i: postnatal day 98 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS28: aortic valve Present 4i* , 4i
TS28: heart valve Present 4i* , 4i
Specimen 4l: postnatal day 98 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS28: visceral pericardium Weak 4lR , 4lR+B , 4lR+B+G
Specimen 4o: postnatal day 98 (more )
Note: Double labeled: red - Mir199a-1 and Mir199a-2; green - Myosin.
Structure Level Pattern Image Note
TS28: endocardial lining Present 4oR+B , 4oR+B+G , 4oR
Specimen 5 right: postnatal day 98 (more )
Note: Triple labeled: red - Mir199a-1 and Mir199a-2; green - Acta2 (assay MGI:7442567); magenta - Col1a1 (assay MGI:7442568) and Col1a2 (assay MGI:7442583).
Structure Cell Type Level Pattern Image Note
TS28: extracellular matrix Present Regionally restricted 5_merge right , 5_miR-199a In the heart ventricle, expression localized in Acta2-negative perivascular cells embedded in the Collagen-I-positive extracellular matrix .
TS28: heart ventricle smooth muscle cell Absent 5_miR-199a , 5_merge right Acta2-positive smooth muscle cells showed no expression.
Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: cardiac muscle tissue cardiac muscle cell Absent
TS22: heart Strong Regionally restricted Strong expression was detected in connective tissue.
TS22: connective tissue Strong Regionally restricted Strong expression was detected in connective tissue of several organs.
Specimen Table 1 P98: postnatal day 98 (more )
Structure Cell Type Level Pattern Image Note
TS28: cardiac muscle tissue cardiac muscle cell Absent
TS28: aorta wall Strong
TS28: aortic valve Strong
TS28: endocardial lining Strong
TS28: heart valve Strong
TS28: visceral pericardium Strong
TS28: artery Strong Regionally restricted Expression was detected in Acta2-negative cells of vessel walls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory