Antibody:	anti-FHL2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: molar enamel organ	Strong		1A
TS22: molar mesenchyme	Strong	Regionally restricted	1A	Expression was detected in the mesenchyme underlying the enamel organ.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present		1B
TS22: molar	Present		1B
TS22: enamel knot	Strong		1B
TS22: inner enamel epithelium	Strong		1B
TS22: outer enamel epithelium	Strong		1B
TS22: stellate reticulum	Strong		1B
TS22: dental papilla	Present		1B
TS22: dental sac	Present		1B

Structure	Level	Pattern	Image	Note
TS24: early bell stage tooth	Present		1D , 1C
TS24: molar	Present		1D , 1C
TS24: inner enamel epithelium	Present		1D , 1C
TS24: outer enamel epithelium	Present		1D , 1C
TS24: secondary enamel knot	Present		1D , 1C
TS24: stratum intermedium	Present		1D , 1C
TS24: stellate reticulum	Absent		1D , 1C
TS24: dental papilla	Present	Regionally restricted	1D , 1C	Positive staining gradually converged at some mesenchymal cells at the cusps of the dental papilla.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: late bell stage tooth		Present		1F , 1E
TS26: molar	ameloblast	Present	Regionally restricted	1F , 1E	Expression was detected in terminal differentiated ameloblasts.
TS26: molar	terminally differentiated odontoblast	Present		1E , 1F
TS26: stratum intermedium		Present		1E , 1F

Structure	Cell Type	Level	Pattern	Image	Note
TS27: molar	ameloblast	Present	Regionally restricted	2A , 2C , 2B	Expression was detected in the secretory and mature ameloblasts.
TS27: molar	odontoblast	Present		2C , 2A , 2B
TS27: enamel		Present		2C , 2A , 2B
TS27: predentin		Absent		2A , 2B , 2C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: molar	ameloblast	Present	Regionally restricted	2E , 2D	Expression was detected in the secretory and mature ameloblasts.
TS28: molar	odontoblast	Present		2D , 2F , 2E
TS28: enamel		Present		2E , 2F , 2D
TS28: dentin		Absent		2E , 2D , 2F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: molar	ameloblast	Present		3A	Expression was detected in mature ameloblasts.
TS28: molar	odontoblast	Present	Regionally restricted	3B , 3A , 3C	Expression was detected at the root furcation.
TS28: Hertwig's epithelial root sheath		Present		3A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: molar epithelium		Present	Regionally restricted	3D	Expression was detected in reduced dental epithelium.
TS28: alveolar process		Present		3F , 3D
TS28: periodontal ligament		Present		3F , 3D
TS28: molar	odontoblast	Present		3E , 3F , 3D	Expression in odontoblasts was detected in both crown and root.
TS28: molar dental pulp	odontoblast	Present	Regionally restricted	3E , 3F , 3D	The only expression in dental pulp was in the odontoblasts.
TS28: blood vessel	blood vessel endothelial cell	Present	Regionally restricted	3E , 3F , 3D