Probe:	pT7CAR
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Present	Regionally restricted	2A	Significant signal was detected in the olfactory bulb surrounding the olfactory ventricle.
TS23: olfactory epithelium	Present		2A

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Widespread	2C
TS26: main olfactory bulb external plexiform layer	Present		2C	Higher levels of expression were seen in the mitral/tufted cell layer relative to the surrounding olfactory bulb.
TS26: main olfactory bulb mitral cell layer	Present		2C	Higher levels of expression were seen in the mitral/tufted cell layer relative to the surrounding olfactory bulb.
TS26: olfactory epithelium	Present		2C

Structure	Cell Type	Level	Pattern	Image	Note
TS27: main olfactory bulb external plexiform layer		Present		2D	Pronounced expression was in the mitral cell layer.
TS27: main olfactory bulb mitral cell layer		Present		2D	Pronounced expression was in the mitral cell layer.
TS27: main olfactory bulb external plexiform layer	olfactory bulb tufted cell	Present		2D	Pronounced expression was in the tufted cell layer.
TS27: main olfactory bulb		Present	Regionally restricted	2D	Pronounced expression was in the juxtaglomerular cell layer.
TS27: olfactory epithelium		Present		2D

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present

Structure	Cell Type	Level	Pattern	Image	Note
TS28: olfactory epithelium	olfactory receptor cell	Present	Regionally restricted	3A	Most of the olfactory sensory neurons showed expression. Robust expression was detected in all the neuronal layers of the olfactory epithelium.
TS28: olfactory epithelium	olfactory epithelial supporting cell	Weak		3A	Much lower expression was detected in sustentacular cells.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Present	Non-Uniform	3B	The expression was similar to that seen at P9. The basal layer of the neuroepithelium displayed occasional regions of higher expression levels.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Weak	Patchy	3C	Patchy expression was dispersed throughout the neuroepithelium. Subsets of cells expressing higher levels were restricted to the deep layer of the neuroepithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: olfactory epithelium	olfactory receptor cell	Absent		3D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb layer		Strong	Widespread	4A	Robust expression was detected in all layers of the olfactory bulb.
TS28: main olfactory bulb glomerular layer		Present		4A
TS28: main olfactory bulb granule cell layer		Present		4A
TS28: main olfactory bulb mitral cell layer		Present		4A
TS28: main olfactory bulb subependymal zone		Present		4A
TS28: main olfactory bulb external plexiform layer	olfactory bulb tufted cell	Present		4A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		4B
TS28: main olfactory bulb granule cell layer	Present		4B